NT5C2 germline variants alter thiopurine metabolism and are associated with acquired NT5C2 relapse mutations in childhood acute lymphoblastic leukaemia

Morten Tulstrup, Marie Grosjean, Stine Nygaard Nielsen, Kathrine Grell, Benjamin Ole Wolthers, Peder Skov Wegener, Olafur Gisli Jonsson, Bendik Lund, Arja Harila-Saari, Jonas Abrahamsson, Goda Vaitkeviciene, Kaie Pruunsild, Nina Toft, Mette Holm, Erik Hulegårdh, Sigurd Liestøl, Laimonas Griskevicius, Mari Punab, Jinhua Wang, William L. CarrollZeyu Zhang, Marlene D. Dalgaard, Ramneek Gupta, Jacob Nersting, Kjeld Schmiegelow

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The antileukaemic drug 6-mercaptopurine is converted into thioguanine nucleotides (TGN) and incorporated into DNA (DNA-TG), the active end metabolite. In a series of genome-wide association studies, we analysed time-weighted means (wm) of erythrocyte concentrations of TGN (Ery-TGN) and DNA-TG in 1009 patients undergoing maintenance therapy for acute lymphoblastic leukaemia (ALL). In discovery analyses (454 patients), the propensity for DNA-TG incorporation (wmDNA-TG/wmEry-TGN ratio) was significantly associated with three intronic SNPs in NT5C2 (top hit: rs72846714; P = 2.09 × 10−10, minor allele frequency 15%). In validation analyses (555 patients), this association remained significant during both early and late maintenance therapy (P = 8.4 × 10−6 and 1.3 × 10−3, respectively). The association was mostly driven by differences in wmEry-TGN, but in regression analyses adjusted for wmEry-TGN (P < 0.0001), rs72846714-A genotype was also associated with a higher wmDNA-TG (P = 0.029). Targeted sequencing of NT5C2 did not identify any missense variants associated with rs72846714 or wmEry-TGN/wmDNA-TG. rs72846714 was not associated with relapse risk, but in a separate cohort of 180 children with relapsed ALL, rs72846714-A genotype was associated with increased occurrence of relapse-specific NT5C2 gain-of-function mutations that reduce cytosol TGN levels (P = 0.03). These observations highlight the impact of both germline and acquired mutations in drug metabolism and disease trajectory.

Original languageEnglish (US)
Pages (from-to)2527-2535
Number of pages9
JournalLeukemia
Volume32
Issue number12
DOIs
StatePublished - Dec 1 2018

Bibliographical note

Funding Information:
Acknowledgements This work was supported by The Danish Cancer Society, The Danish Childhood Cancer Foundation, The Swedish Childhood Cancer Foundation, The Nordic Cancer Union, The Otto Christensen Foundation, University Hospital Rigshospitalet and The Novo Nordic Foundation.

Fingerprint Dive into the research topics of 'NT5C2 germline variants alter thiopurine metabolism and are associated with acquired NT5C2 relapse mutations in childhood acute lymphoblastic leukaemia'. Together they form a unique fingerprint.

Cite this