Novel synthesis and characterization of a collagen-based biopolymer initiated by hydroxyapatite nanoparticles

D. Bhuiyan, M. J. Jablonsky, I. Kolesov, J. Middleton, T. M. Wick, R. Tannenbaum

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


In this study, we developed a novel synthesis method to create a complex collagen-based biopolymer that promises to possess the necessary material properties for a bone graft substitute. The synthesis was carried out in several steps. In the first step, a ring-opening polymerization reaction initiated by hydroxyapatite nanoparticles was used to polymerize d,l-lactide and glycolide monomers to form poly(lactide-co-glycolide) co-polymer. In the second step, the polymerization product was coupled with succinic anhydride, and subsequently was reacted with N-hydroxysuccinimide in the presence of dicyclohexylcarbodiimide as the cross-linking agent, in order to activate the co-polymer for collagen attachment. In the third and final step, the activated co-polymer was attached to calf skin collagen type I, in hydrochloric acid/phosphate buffer solution and the precipitated co-polymer with attached collagen was isolated. The synthesis was monitored by proton nuclear magnetic resonance, infrared and Raman spectroscopies, and the products after each step were characterized by thermal and mechanical analysis. Calculations of the relative amounts of the various components, coupled with initial dynamic mechanical analysis testing of the resulting biopolymer, afforded a preliminary assessment of the structure of the complex biomaterial formed by this novel polymerization process.

Original languageEnglish (US)
Pages (from-to)181-190
Number of pages10
JournalActa Biomaterialia
StatePublished - Mar 15 2015

Bibliographical note

Funding Information:
The authors acknowledge the financial support of the School of Engineering at the University of Alabama at Birmingham and the valuable discussions with Drs. Hamid Garmestani Georgia Tech, Paul Eleazer and Eugenia Kharlampieva of UAB.

Publisher Copyright:
© 2015 Published by Elsevier Ltd. on behalf of Acta Materialia Inc.


  • Biodegradable polymer
  • Collagen
  • Hydroxyapatite
  • PLGA


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