Novel strategy for production of aggregation-prone proteins and lytic enzymes in Escherichia coli based on an anchored periplasmic expression system

Keyphrases

• Escherichia Coli 100%
• Expression System 100%
• Inner Membrane 100%
• Protein Enzymes 100%
• Lytic Enzymes 100%
• Aggregation-prone Protein 100%
• Periplasmic Expression 100%
• Protein Aggregation 50%
• Recombinant Protein 50%
• Periplasm 50%
• Lytic Activity 50%
• Efficient Production 25%
• Cell Lysis 25%
• Membrane Protein 25%
• Protein Accumulation 25%
• Model Proteins 25%
• Lipase 25%
• Bioactivity 25%
• Purification Process 25%
• Serum Leptin 25%
• Heterologous Protein 25%
• High Purity 25%
• High Production 25%
• Recombinant Protein Production 25%
• Aggregation Behavior 25%
• Pseudomonas Fluorescens 25%
• Inclusion Bodies 25%
• Production Yield 25%
• Simple Purification 25%
• Aggregation-prone 25%
• Optimized Culture Conditions 25%

Biochemistry, Genetics and Molecular Biology

• Hydrolase 100%
• Escherichia coli 100%
• Gene Expression System 100%
• Inner Membrane 100%
• Recombinant Protein 75%
• Protein Aggregation 50%
• Periplasm 50%
• Copurification 25%
• Protein Expression 25%
• Cytolysis 25%
• Biological Activity 25%
• Pseudomonas fluorescens 25%
• Triacylglycerol Lipase 25%
• Pancreatic Lipase 25%
• Leptin 25%

Immunology and Microbiology

• Escherichia coli 100%
• Gene Expression System 100%
• Inner Membrane 100%
• Periplasmic Space 50%
• Protein Aggregation 50%
• Inclusion Bodies 25%
• Protein Expression 25%
• Biological Activity 25%
• Cytolysis 25%
• Pseudomonas fluorescens 25%