TY - JOUR
T1 - Novel strategy for measuring creatine kinase reaction rate in the in vivo heart
AU - Xiong, Qiang
AU - Li, Qinglu
AU - Mansoor, Abdul
AU - Jameel, Mohammad Nurulqadr
AU - Du, Fei
AU - Chen, Wei
AU - Zhang, Jianyi
PY - 2009/9
Y1 - 2009/9
N2 - In the heart, the creatine kinase (CK) system plays an important role in the cascade of ATP production, transportation, and utilization. The forward pseudo-first-order rate constant for the CK reaction can be measured noninvasively by the 31P-magnetic resonance (MR) spectroscopy magnetization saturation transfer (MST) techniques. However, the measurement of MST in the in vivo heart is limited by the lengthy data acquisition time, especially for studies requiring spatial localization. This technical report presents a new method for measuring ATP production rate via CK that can reduce the MST data acquisition time by 82%. This method is validated using an in vivo pig model to evaluate the forward pseudo-first-order rate constant of myocardial CK reaction noninvasively.
AB - In the heart, the creatine kinase (CK) system plays an important role in the cascade of ATP production, transportation, and utilization. The forward pseudo-first-order rate constant for the CK reaction can be measured noninvasively by the 31P-magnetic resonance (MR) spectroscopy magnetization saturation transfer (MST) techniques. However, the measurement of MST in the in vivo heart is limited by the lengthy data acquisition time, especially for studies requiring spatial localization. This technical report presents a new method for measuring ATP production rate via CK that can reduce the MST data acquisition time by 82%. This method is validated using an in vivo pig model to evaluate the forward pseudo-first-order rate constant of myocardial CK reaction noninvasively.
KW - Adenosine 5′-triphosphate
KW - High-energy phosphates
KW - Magnetic resonance spectroscopy
KW - Metabolism
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U2 - 10.1152/ajpheart.01195.2008
DO - 10.1152/ajpheart.01195.2008
M3 - Article
C2 - 19561307
AN - SCOPUS:69249157341
SN - 0363-6135
VL - 297
SP - H1010-H1019
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
IS - 3
ER -