Novel strategy for measuring creatine kinase reaction rate in the in vivo heart

Qiang Xiong, Qinglu Li, Abdul Mansoor, Mohammad Nurulqadr Jameel, Fei Du, Wei Chen, Jianyi Zhang

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

In the heart, the creatine kinase (CK) system plays an important role in the cascade of ATP production, transportation, and utilization. The forward pseudo-first-order rate constant for the CK reaction can be measured noninvasively by the 31P-magnetic resonance (MR) spectroscopy magnetization saturation transfer (MST) techniques. However, the measurement of MST in the in vivo heart is limited by the lengthy data acquisition time, especially for studies requiring spatial localization. This technical report presents a new method for measuring ATP production rate via CK that can reduce the MST data acquisition time by 82%. This method is validated using an in vivo pig model to evaluate the forward pseudo-first-order rate constant of myocardial CK reaction noninvasively.

Original languageEnglish (US)
Pages (from-to)H1010-H1019
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume297
Issue number3
DOIs
StatePublished - Sep 2009

Keywords

  • Adenosine 5′-triphosphate
  • High-energy phosphates
  • Magnetic resonance spectroscopy
  • Metabolism

Fingerprint Dive into the research topics of 'Novel strategy for measuring creatine kinase reaction rate in the in vivo heart'. Together they form a unique fingerprint.

Cite this