Novel phosphoinositides in barley aleurone cells

Additional evidence for the presence of phosphatidyl-scyllo-inositol

Bhuvarahamurthy Narasimhan, Gay Pliska-Matyshak, Richard Kinnard, Sven Carstensen, Michele A. Ritter, Linda Von Weymarn, Pushpalatha P.N. Murthy

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A novel isomer of phosphatidylinositol that differs in the structure of the head group was detected in barley (Hordeurn vulgate cv Himalaya) seeds. In this paper we describe our efforts to elucidate the structure of the novel isomer. Evidence from a variety of techniques, including chemical modification of in vivo 32Pi- and myo-[3H]inositol-labeled compounds, gas chromatography-mass spectrometry analysis, in vivo incorporation of scyllo-[3H]inositol, and enzymatic studies that suggest that the structure is phosphatidylscyllo-inositol (scyllo-PI), is presented. The use of microwave energy to significantly enhance the slow rate of hydrolysis of phosphoinositides is described. The presence of scyllo-PI can be easily overlooked by the methods commonly employed; therefore, experimental considerations important for the detection of scyllo-PI are discussed.

Original languageEnglish (US)
Pages (from-to)1385-1393
Number of pages9
JournalPlant physiology
Volume113
Issue number4
DOIs
StatePublished - Jan 1 1997

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aleurone cells
Hordeum
Phosphatidylinositols
barley
isomers
Inositol
Microwaves
Gas Chromatography-Mass Spectrometry
Seeds
Hydrolysis
phosphatidylinositols
hydrolysis
energy
seeds
methodology
scyllitol
inositols

Cite this

Narasimhan, B., Pliska-Matyshak, G., Kinnard, R., Carstensen, S., Ritter, M. A., Von Weymarn, L., & Murthy, P. P. N. (1997). Novel phosphoinositides in barley aleurone cells: Additional evidence for the presence of phosphatidyl-scyllo-inositol. Plant physiology, 113(4), 1385-1393. https://doi.org/10.1104/pp.113.4.1385

Novel phosphoinositides in barley aleurone cells : Additional evidence for the presence of phosphatidyl-scyllo-inositol. / Narasimhan, Bhuvarahamurthy; Pliska-Matyshak, Gay; Kinnard, Richard; Carstensen, Sven; Ritter, Michele A.; Von Weymarn, Linda; Murthy, Pushpalatha P.N.

In: Plant physiology, Vol. 113, No. 4, 01.01.1997, p. 1385-1393.

Research output: Contribution to journalArticle

Narasimhan, B, Pliska-Matyshak, G, Kinnard, R, Carstensen, S, Ritter, MA, Von Weymarn, L & Murthy, PPN 1997, 'Novel phosphoinositides in barley aleurone cells: Additional evidence for the presence of phosphatidyl-scyllo-inositol', Plant physiology, vol. 113, no. 4, pp. 1385-1393. https://doi.org/10.1104/pp.113.4.1385
Narasimhan, Bhuvarahamurthy ; Pliska-Matyshak, Gay ; Kinnard, Richard ; Carstensen, Sven ; Ritter, Michele A. ; Von Weymarn, Linda ; Murthy, Pushpalatha P.N. / Novel phosphoinositides in barley aleurone cells : Additional evidence for the presence of phosphatidyl-scyllo-inositol. In: Plant physiology. 1997 ; Vol. 113, No. 4. pp. 1385-1393.
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