Novel peroxiredoxin-based sensor for sensitive detection of hydrogen peroxide

Yuhong Yang; Yao Zhou; Jingyi Li; Haijun Yu; N. Takaya; Ping Wang; Shengmin Zhou

doi:10.1016/j.bbrc.2019.07.062

Novel peroxiredoxin-based sensor for sensitive detection of hydrogen peroxide

Yuhong Yang, Yao Zhou, Jingyi Li, Haijun Yu, N. Takaya, Ping Wang, Shengmin Zhou

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A series of genetically encoded sensors have been developed to detect the important signaling molecule H₂O₂ in living cells. However, more responsive and sensitive biosensors need to be developed. To address these demands, we used E. coli as a platform to develop a novel fluorescent H₂O₂ sensor, which we refer to as TScGP. This sensor employs a circularly permuted YFP (cpYFP) and is based on a redox relay between peroxiredoxin (Prx) and thioredoxin (Trx). Structurally, cpYFP is sandwiched between a fungal PrxA and a C-terminal cysteine mutated TrxA that can form a stabilized disulfide bond between PrxA and TrxA in response to H₂O₂. We confirmed that TScGP can be used for detecting exogenous H₂O₂ in the range of 0.5–5 μM with high selectivity and rapidly detecting H₂O₂ within 30 s in E. coli. To demonstrate an application, cellular H₂O₂ production by menadione was detected directly by TScGP. Our results demonstrated that using Prx-Trx combination as a sensing moiety is another strategy in designing H₂O₂ sensor with high performance.

Original language	English (US)
Pages (from-to)	260-265
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	517
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2019.07.062
State	Published - Sep 17 2019
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank financial support for this work from National Science Foundation of China ( 21672065 and 21636003 ), the Fundamental Research Funds for the Central Universities ( 22221818014 ), the 111 Project ( B18022 ),the Project Funded by the International S&T Innovation Cooperation Key Project ( 2017YFE0129600 ), and Shanghai Pujiang Program ( 16PJ1402500 ).

Publisher Copyright:
© 2019 Elsevier Inc.

Keywords

Biosensor
Hydrogen peroxide
Peroxiredoxin
Sensitive detection
Thioredoxin

Publisher link

10.1016/j.bbrc.2019.07.062

Find It

Find It at U of M Libraries

Cite this

@article{648e01d425534a33846c125d4c219a6e,

title = "Novel peroxiredoxin-based sensor for sensitive detection of hydrogen peroxide",

abstract = "A series of genetically encoded sensors have been developed to detect the important signaling molecule H2O2 in living cells. However, more responsive and sensitive biosensors need to be developed. To address these demands, we used E. coli as a platform to develop a novel fluorescent H2O2 sensor, which we refer to as TScGP. This sensor employs a circularly permuted YFP (cpYFP) and is based on a redox relay between peroxiredoxin (Prx) and thioredoxin (Trx). Structurally, cpYFP is sandwiched between a fungal PrxA and a C-terminal cysteine mutated TrxA that can form a stabilized disulfide bond between PrxA and TrxA in response to H2O2. We confirmed that TScGP can be used for detecting exogenous H2O2 in the range of 0.5–5 μM with high selectivity and rapidly detecting H2O2 within 30 s in E. coli. To demonstrate an application, cellular H2O2 production by menadione was detected directly by TScGP. Our results demonstrated that using Prx-Trx combination as a sensing moiety is another strategy in designing H2O2 sensor with high performance.",

keywords = "Biosensor, Hydrogen peroxide, Peroxiredoxin, Sensitive detection, Thioredoxin",

author = "Yuhong Yang and Yao Zhou and Jingyi Li and Haijun Yu and N. Takaya and Ping Wang and Shengmin Zhou",

note = "Funding Information: The authors thank financial support for this work from National Science Foundation of China ( 21672065 and 21636003 ), the Fundamental Research Funds for the Central Universities ( 22221818014 ), the 111 Project ( B18022 ),the Project Funded by the International S&T Innovation Cooperation Key Project ( 2017YFE0129600 ), and Shanghai Pujiang Program ( 16PJ1402500 ). Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

year = "2019",

month = sep,

day = "17",

doi = "10.1016/j.bbrc.2019.07.062",

language = "English (US)",

volume = "517",

pages = "260--265",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Novel peroxiredoxin-based sensor for sensitive detection of hydrogen peroxide

AU - Yang, Yuhong

AU - Zhou, Yao

AU - Li, Jingyi

AU - Yu, Haijun

AU - Takaya, N.

AU - Wang, Ping

AU - Zhou, Shengmin

N1 - Funding Information: The authors thank financial support for this work from National Science Foundation of China ( 21672065 and 21636003 ), the Fundamental Research Funds for the Central Universities ( 22221818014 ), the 111 Project ( B18022 ),the Project Funded by the International S&T Innovation Cooperation Key Project ( 2017YFE0129600 ), and Shanghai Pujiang Program ( 16PJ1402500 ). Publisher Copyright: © 2019 Elsevier Inc.

PY - 2019/9/17

Y1 - 2019/9/17

N2 - A series of genetically encoded sensors have been developed to detect the important signaling molecule H2O2 in living cells. However, more responsive and sensitive biosensors need to be developed. To address these demands, we used E. coli as a platform to develop a novel fluorescent H2O2 sensor, which we refer to as TScGP. This sensor employs a circularly permuted YFP (cpYFP) and is based on a redox relay between peroxiredoxin (Prx) and thioredoxin (Trx). Structurally, cpYFP is sandwiched between a fungal PrxA and a C-terminal cysteine mutated TrxA that can form a stabilized disulfide bond between PrxA and TrxA in response to H2O2. We confirmed that TScGP can be used for detecting exogenous H2O2 in the range of 0.5–5 μM with high selectivity and rapidly detecting H2O2 within 30 s in E. coli. To demonstrate an application, cellular H2O2 production by menadione was detected directly by TScGP. Our results demonstrated that using Prx-Trx combination as a sensing moiety is another strategy in designing H2O2 sensor with high performance.

AB - A series of genetically encoded sensors have been developed to detect the important signaling molecule H2O2 in living cells. However, more responsive and sensitive biosensors need to be developed. To address these demands, we used E. coli as a platform to develop a novel fluorescent H2O2 sensor, which we refer to as TScGP. This sensor employs a circularly permuted YFP (cpYFP) and is based on a redox relay between peroxiredoxin (Prx) and thioredoxin (Trx). Structurally, cpYFP is sandwiched between a fungal PrxA and a C-terminal cysteine mutated TrxA that can form a stabilized disulfide bond between PrxA and TrxA in response to H2O2. We confirmed that TScGP can be used for detecting exogenous H2O2 in the range of 0.5–5 μM with high selectivity and rapidly detecting H2O2 within 30 s in E. coli. To demonstrate an application, cellular H2O2 production by menadione was detected directly by TScGP. Our results demonstrated that using Prx-Trx combination as a sensing moiety is another strategy in designing H2O2 sensor with high performance.

KW - Biosensor

KW - Hydrogen peroxide

KW - Peroxiredoxin

KW - Sensitive detection

KW - Thioredoxin

UR - http://www.scopus.com/inward/record.url?scp=85069659603&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85069659603&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2019.07.062

DO - 10.1016/j.bbrc.2019.07.062

M3 - Article

C2 - 31349967

AN - SCOPUS:85069659603

SN - 0006-291X

VL - 517

SP - 260

EP - 265

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Novel peroxiredoxin-based sensor for sensitive detection of hydrogen peroxide

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this