Novel Mouse Model of Murine Cytomegalovirus-Induced Adaptive NK Cells

Isaac J. Jensen, Matthew D. Martin, Sandeep K. Tripathy, Vladimir P. Badovinac

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

NK cells are important mediators of viral control with the capacity to form adaptive immune features following infection. However, studies of infection-induced adaptive NK cells require adoptive cell transfer to lower the precursor frequency of .Ag-specific. NK cells, potentially limiting the diversity of the NK cell response. In seeking an unmanipulated model to probe the adaptive NK cells, we interrogated a wide range of Collaborative Cross (CC) inbred mice, inbred mouse strains that exhibit broad genetic diversity across strains. Our assessment identified and validated a putative .ideal. CC strain, CC006, which does not require manipulation to generate and maintain adaptive NK cells. Critically, CC006 mice, in contrast to C57BL/6 mice, are capable of developing enhanced NK cell-mediated protective responses to murine CMV infection following m157-mediated vaccination. This work both furthers our understanding of adaptive NK cells and demonstrates the utility of CC mice in the development and interrogation of immunologic models.

Original languageEnglish (US)
Pages (from-to)8-15
Number of pages8
JournalImmunoHorizons
Volume6
Issue number1
DOIs
StatePublished - Jan 1 2022

Bibliographical note

Funding Information:
Received for publication December 13, 2021. Accepted for publication December 13, 2021. Address correspondence and reprint requests to: Dr. Isaac J. Jensen, Columbia University Irving Medical Center, 1313 Hammer Health Sciences Building, 701 W 168th Street, New York, NY 10032. E-mail address: ijj2106@cumc.columbia.edu ORCIDs: 0000-0002-3107-3961 (I.J.J.); 0000-0003-3180-2439 (V.P.B.). This work was supported by National Institutes of Health Grants T32AI007511 (to I.J.J.), T32AI007485 (to I.J.J.), 5R01AI114543 (to V.P.B.), and 1R35GM134880 (to V.P.B.). Abbreviations used in this article: B6, C57BL/6; B6-m157, B6 mice with transgenic expression of m157; CC, Collaborative Cross; CC006, CC006/TauUncJ; MCMV, murine CMV. The online version of this article contains supplemental material. This article is distributed under the terms of the CC BY 4.0 Unported license.

Publisher Copyright:
© 2022 American Association of Immunologists. All rights reserved.

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