Novel method for transdiaphragmatic pressure measurements in mice

Sarah M. Greising, Dylan C. Sieck, Gary C. Sieck, Carlos B. Mantilla

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


The diaphragm muscle (DIAm) is responsible for breathing and determines the ability to generate both ventilatory and non-ventilatory behaviors. Size limitations of the mouse make transdiaphragmatic pressure (Pdi) measurement using a dual balloon system untenable. Adult C57BL/6J mice (n=8) and C57BL/6×129 (n=9), underwent Pdi measurements using solid-state pressure catheters spanning the thoracic and abdominal surfaces of the DIAm. Measurements were conducted during eupnea, hypoxia (10% O2)-hypercapnia (5% CO2), chemical airway stimulation (i.e., sneezing), spontaneously occurring deep breaths, sustained tracheal occlusion, and bilateral phrenic nerve stimulation. There was a difference in the Pdi generated across the range of ventilatory and non-ventilatory behaviors (p=0.001). No difference in Pdi across behaviors was evident between mouse strains (p=0.161). This study establishes a novel method to determine Pdi across a range of DIAm behaviors in mice that may be useful in evaluating conditions associated with reduced ability to perform expulsive, non-ventilatory behaviors.

Original languageEnglish (US)
Pages (from-to)56-59
Number of pages4
JournalRespiratory Physiology and Neurobiology
Issue number1
StatePublished - Aug 1 2013

Bibliographical note

Funding Information:
We would like to acknowledge Barry J. Kenny and Juan S. Medina for technical assistance in the completion of this project. This research was supported by grants from National Institute of Health RO1-HL096750 (C.B.M. and G.C.S.) and T32-HL105355 (S.M.G.), and the Mayo Clinic .


  • Diaphragm muscle
  • Mouse strain
  • Phrenic nerve
  • Respiratory compliance


Dive into the research topics of 'Novel method for transdiaphragmatic pressure measurements in mice'. Together they form a unique fingerprint.

Cite this