Abstract
The concentration of 5-hydroxymethlfurfural (5-HMF) in glucose biphasic hydrolysate is rapidly determined by UV spectroscopy. With 5-HMF as the standard sample and the distilled water as the controlling blank instead of water-dimethylsulfoxide (DMSO) biphasic solution, the absorbance of 5-HMF at 284 nm is analyzed. The results show that a favorable linear relationship between the absorbance and the concentration of 5-HMF can be obtained with good reproducibility when the concentration of 5-HMF is within the range of 0-0.10 mmol/L. This method is suitable for rapid and accurate determination of 5-HMF.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 92-95 |
| Number of pages | 4 |
| Journal | Xiandai Huagong/Modern Chemical Industry |
| Volume | 31 |
| Issue number | 10 |
| State | Published - Oct 1 2011 |
Keywords
- 5-hydroxymethlfurfural (5-HMF)
- Glucose biphasic hydrolysate
- UV spectroscopy