Novel in situ collection of tumor interstitial fluid from a head and neck squamous carcinoma reveals a unique proteome with diagnostic potential

Matthew D. Stone, Rick M. Odland, Thomas McGowan, Getiria Onsongo, Chaunning Tang, Nelson L. Rhodus, Pratik Jagtap, Sricharan Bandhakavi, Timothy J. Griffin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Introduction: Tumors lack normal drainage of secreted fluids and consequently build up tumor interstitial fluid (TIF). Unlike other bodily fluids, TIF likely contains a high proportion of tumor-specific proteins with potential as biomarkers. Methods: Here, we evaluated a novel technique using a unique ultrafiltration catheter for in situ collection of TIF and used it to generate the first catalog of TIF proteins from a head and neck squamous cell carcinoma (HNSCC). To maximize proteomic coverage, TIF was immunodepleted for high abundance proteins and digested with trypsin, and peptides were fractionated in three dimensions prior to mass spectrometry. Results: We identified 525 proteins with high confidence. The HNSCC TIF proteome was distinct compared to proteomes of other bodily fluids. It contained a relatively high proportion of proteins annotated by Gene Ontology as " extracellular" compared to other secreted fluid and cellular proteomes, indicating minimal cell lysis from our in situ collection technique. Several proteins identified are putative biomarkers of HNSCC, supporting our catalog's value as a source of potential biomarkers. Conclusions: In all, we demonstrate a reliable new technique for in situ TIF collection and provide the first HNSCC TIF protein catalog with value as a guide for others seeking to develop tumor biomarkers.

Original languageEnglish (US)
Pages (from-to)75-82
Number of pages8
JournalClinical Proteomics
Volume6
Issue number3
DOIs
StatePublished - Sep 1 2010

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Extracellular Fluid
Proteome
Tumors
Squamous Cell Carcinoma
Neck
Head
Fluids
Neoplasms
Proteins
Biomarkers
Gene Ontology
Ultrafiltration
Tumor Biomarkers
Catheters
Proteomics
Trypsin
Drainage
Mass Spectrometry
Mass spectrometry
Ontology

Keywords

  • Biomarker discovery
  • Head and neck squamous cell carcinoma
  • In situ collection
  • Tumor interstitial fluid

Cite this

Novel in situ collection of tumor interstitial fluid from a head and neck squamous carcinoma reveals a unique proteome with diagnostic potential. / Stone, Matthew D.; Odland, Rick M.; McGowan, Thomas; Onsongo, Getiria; Tang, Chaunning; Rhodus, Nelson L.; Jagtap, Pratik; Bandhakavi, Sricharan; Griffin, Timothy J.

In: Clinical Proteomics, Vol. 6, No. 3, 01.09.2010, p. 75-82.

Research output: Contribution to journalArticle

Stone, Matthew D. ; Odland, Rick M. ; McGowan, Thomas ; Onsongo, Getiria ; Tang, Chaunning ; Rhodus, Nelson L. ; Jagtap, Pratik ; Bandhakavi, Sricharan ; Griffin, Timothy J. / Novel in situ collection of tumor interstitial fluid from a head and neck squamous carcinoma reveals a unique proteome with diagnostic potential. In: Clinical Proteomics. 2010 ; Vol. 6, No. 3. pp. 75-82.
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