Novel florfenicol and chloramphenicol resistance gene discovered in alaskan soil by using functional metagenomics

Kevin S. Lang; Janet M. Anderson; Stefan Schwarz; Lynn Williamson; Jo Handelsman; Randall S. Singer

doi:10.1128/AEM.00323-10

Novel florfenicol and chloramphenicol resistance gene discovered in alaskan soil by using functional metagenomics

Kevin S. Lang
, Janet M. Anderson
, Stefan Schwarz
, Lynn Williamson
, Jo Handelsman
, Randall S. Singer

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

Functional metagenomics was used to search for florfenicol resistance genes in libraries of cloned DNA isolated from Alaskan soil. A gene that mediated reduced susceptibility to florfenicol was identified and designated pexA. The predicted PexA protein showed a structure similar to that of efflux pumps of the major facilitator superfamily.

Original language	English (US)
Pages (from-to)	5321-5326
Number of pages	6
Journal	Applied and environmental microbiology
Volume	76
Issue number	15
DOIs	https://doi.org/10.1128/AEM.00323-10
State	Published - Aug 2010

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title = "Novel florfenicol and chloramphenicol resistance gene discovered in alaskan soil by using functional metagenomics",

abstract = "Functional metagenomics was used to search for florfenicol resistance genes in libraries of cloned DNA isolated from Alaskan soil. A gene that mediated reduced susceptibility to florfenicol was identified and designated pexA. The predicted PexA protein showed a structure similar to that of efflux pumps of the major facilitator superfamily.",

author = "Lang, \{Kevin S.\} and Anderson, \{Janet M.\} and Stefan Schwarz and Lynn Williamson and Jo Handelsman and Singer, \{Randall S.\}",

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AU - Lang, Kevin S.

AU - Anderson, Janet M.

AU - Schwarz, Stefan

AU - Williamson, Lynn

AU - Handelsman, Jo

AU - Singer, Randall S.

PY - 2010/8

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AB - Functional metagenomics was used to search for florfenicol resistance genes in libraries of cloned DNA isolated from Alaskan soil. A gene that mediated reduced susceptibility to florfenicol was identified and designated pexA. The predicted PexA protein showed a structure similar to that of efflux pumps of the major facilitator superfamily.

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UR - https://www.scopus.com/pages/publications/77955562279#tab=citedBy

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AN - SCOPUS:77955562279

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JO - Applied and environmental microbiology

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ER -

Novel florfenicol and chloramphenicol resistance gene discovered in alaskan soil by using functional metagenomics

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