Bats from the families Rhinolophidae (n = 90) and Vespertilionidae (n = 191) in the USA and Czech Republic were screened for the presence of Cryptosporidium by microscopic and molecular analysis of faecal samples collected from rectum of dissected animals and from the ground beneath roosting sites. Cryptosporidium oocysts were not detected in any of the 281 faecal specimens examined using the aniline–carbol–methyl violet staining method. Nested PCR amplification, sequencing and phylogenetic analysis of the small ribosomal subunit rRNA and actin genes were used to identify isolates and infer evolutionary relationships. Cryptosporidium parvum was identified in a western small-footed bat (Myotis ciliolabrum) from the USA and a common pipistrelle bats (Pipistrellus pipistrellus) from the Czech Republic. Two novel genotypes were identified and named Cryptosporidium bat genotype III and IV. Bat genotype III was found in two big brown bats (Eptesicus fuscus) from the USA. Bat genotype IV was detected in two common pipistrelle bats from the Czech Republic.
Bibliographical noteFunding Information:
This study was funded by projects of the Czech Science Foundation (15-01090S), the Grant Agency of University of South Bohemia (011/2013/Z), United States Department of Agriculture National Research Initiative (Project # 2008-35102-19260) and MEYS of the Czech Republic under the NPU I program (LO1218). The authors thank Michal Andreas, Pavel Benda, Lenka Florková, Daniel Horáček, Josef Hotový, Antonín Reiter, Zdeněk Řehák, Jiří Šafář and Magda Timplová for help with sample collection.
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