Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids

Ivana Drienovská; Ana Rioz-Martínez; Apparao Draksharapu; Gerard Roelfes

doi:10.1039/c4sc01525h

Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids

Ivana Drienovská
, Ana Rioz-Martínez
, Apparao Draksharapu
, Gerard Roelfes

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

173 Scopus citations

Abstract

Artificial metalloenzymes have emerged as an attractive new approach to enantioselective catalysis. Herein, we introduce a novel strategy for preparation of artificial metalloenzymes utilizing amber stop codon suppression methodology for the in vivo incorporation of metal-binding unnatural amino acids. The resulting artificial metalloenzymes were applied in catalytic asymmetric Friedel-Crafts alkylation reactions and up to 83% ee for the product was achieved.

Original language	English (US)
Pages (from-to)	770-776
Number of pages	7
Journal	Chemical Science
Volume	6
Issue number	1
DOIs	https://doi.org/10.1039/c4sc01525h
State	Published - Jan 1 2015

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© 2015 The Royal Society of Chemistry.

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abstract = "Artificial metalloenzymes have emerged as an attractive new approach to enantioselective catalysis. Herein, we introduce a novel strategy for preparation of artificial metalloenzymes utilizing amber stop codon suppression methodology for the in vivo incorporation of metal-binding unnatural amino acids. The resulting artificial metalloenzymes were applied in catalytic asymmetric Friedel-Crafts alkylation reactions and up to 83\% ee for the product was achieved.",

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Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids

Abstract

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