Novel 3D-Printed Microfluidic Magnetic Platform for Rapid DNA Isolation

Güneş Kibar; Büşra Sarıarslan; Serkan Doğanay; Gökay Yıldız; O. Berk Usta; Barbaros Çetin

doi:10.1021/acs.analchem.3c04412

Novel 3D-Printed Microfluidic Magnetic Platform for Rapid DNA Isolation

Güneş Kibar, Büşra Sarıarslan, Serkan Doğanay, Gökay Yıldız, O. Berk Usta, Barbaros Çetin

Advanced Technologies for Preservation of Biological Systems

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

This study presents a novel miniaturized device as a 3D-printed microfluidic magnetic platform specifically designed to manipulate magnetic microparticles in a microfluidic chip for rapid deoxyribonucleic acid (DNA) isolation. The novel design enables the movement of the magnetic particles in the same or opposite directions with the flow or suspends them in continuous flow. A computational model was developed to assess the effectiveness of the magnetic manipulation of the particles. Superparamagnetic monodisperse silica particles synthesized in-house are utilized for the isolation of fish sperm DNA and human placenta DNA. It was demonstrated that the proposed platform can perform DNA isolation within 10 min with an isolation efficiency of 50% at optimum operating conditions.

Original language	English (US)
Pages (from-to)	1985-1992
Number of pages	8
Journal	Analytical Chemistry
Volume	96
Issue number	5
DOIs	https://doi.org/10.1021/acs.analchem.3c04412
State	Published - Feb 6 2024

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© 2024 American Chemical Society.

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10.1021/acs.analchem.3c04412

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title = "Novel 3D-Printed Microfluidic Magnetic Platform for Rapid DNA Isolation",

abstract = "This study presents a novel miniaturized device as a 3D-printed microfluidic magnetic platform specifically designed to manipulate magnetic microparticles in a microfluidic chip for rapid deoxyribonucleic acid (DNA) isolation. The novel design enables the movement of the magnetic particles in the same or opposite directions with the flow or suspends them in continuous flow. A computational model was developed to assess the effectiveness of the magnetic manipulation of the particles. Superparamagnetic monodisperse silica particles synthesized in-house are utilized for the isolation of fish sperm DNA and human placenta DNA. It was demonstrated that the proposed platform can perform DNA isolation within 10 min with an isolation efficiency of 50% at optimum operating conditions.",

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AU - Kibar, Güneş

AU - Sarıarslan, Büşra

AU - Doğanay, Serkan

AU - Yıldız, Gökay

AU - Usta, O. Berk

AU - Çetin, Barbaros

PY - 2024/2/6

Y1 - 2024/2/6

N2 - This study presents a novel miniaturized device as a 3D-printed microfluidic magnetic platform specifically designed to manipulate magnetic microparticles in a microfluidic chip for rapid deoxyribonucleic acid (DNA) isolation. The novel design enables the movement of the magnetic particles in the same or opposite directions with the flow or suspends them in continuous flow. A computational model was developed to assess the effectiveness of the magnetic manipulation of the particles. Superparamagnetic monodisperse silica particles synthesized in-house are utilized for the isolation of fish sperm DNA and human placenta DNA. It was demonstrated that the proposed platform can perform DNA isolation within 10 min with an isolation efficiency of 50% at optimum operating conditions.

AB - This study presents a novel miniaturized device as a 3D-printed microfluidic magnetic platform specifically designed to manipulate magnetic microparticles in a microfluidic chip for rapid deoxyribonucleic acid (DNA) isolation. The novel design enables the movement of the magnetic particles in the same or opposite directions with the flow or suspends them in continuous flow. A computational model was developed to assess the effectiveness of the magnetic manipulation of the particles. Superparamagnetic monodisperse silica particles synthesized in-house are utilized for the isolation of fish sperm DNA and human placenta DNA. It was demonstrated that the proposed platform can perform DNA isolation within 10 min with an isolation efficiency of 50% at optimum operating conditions.

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Novel 3D-Printed Microfluidic Magnetic Platform for Rapid DNA Isolation

Abstract

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ATP-Bio: NSF Engineering Research Center for Advanced Technologies for the Preservation of Biological Systems (ATP-Bio)

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Novel 3D-Printed Microfluidic Magnetic Platform for Rapid DNA Isolation

Abstract

Bibliographical note

PubMed: MeSH publication types

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Projects

ATP-Bio: NSF Engineering Research Center for Advanced Technologies for the Preservation of Biological Systems (ATP-Bio)

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