An ideal synthetic bone graft is a combination of the porous and nanofibrous structure presented by natural bone tissue as well as osteoinductive biochemical factors such as bone morphogenetic protein 2 (BMP-2). In this work, ultralight 3D hybrid nanofiber aerogels composed of electrospun PLGA-collagen-gelatin and Sr–Cu codoped bioactive glass fibers with incorporation of heptaglutamate E7 domain specific BMP-2 peptides have been developed and evaluated for their potential in cranial bone defect healing. The nanofiber aerogels are surgically implanted into 8 mm × 1 mm (diameter × thickness) critical-sized defects created in rat calvariae. A sustained release of E7-BMP-2 peptide from the degradable hybrid aerogels significantly enhances bone healing and defect closure over 8 weeks in comparison to unfilled defects. Histomorphometry and X-ray microcomputed tomography (µ-CT) analysis reveal greater bone volume and bone formation area in case of the E7-BMP-2 peptide loaded hybrid nanofiber aerogels. Further, histopathology data divulged a near complete nanofiber aerogel degradation along with enhanced vascularization of the regenerated tissue. Together, this study for the first time demonstrates the fabrication of 3D hybrid nanofiber aerogels from 2D electrospun fibers and their loading with therapeutic osteoinductive BMP-2 mimicking peptide for cranial bone tissue regeneration.
Bibliographical noteFunding Information:
This work was supported by the grant from the National Institute of General Medical Science (NIGMS) at National Institute of Health (NIH) (2P20 GM103480-06), Nebraska Regenerative Medicine Program Pilot Project Grant, and startup funds from the University of Nebraska Medical Center.
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Copyright 2020 Elsevier B.V., All rights reserved.
- bioactive glass (BG) nanofibers
- bone morphogenetic protein (BMP-2) mimicking peptide
- cranial bone regeneration
- hybrid nanofiber aerogel