Noninvasive tumor oxygen imaging by photoacoustic lifetime imaging integrated with photodynamic therapy

Qi Shao, Merrill A. Biel, Shai Ashkenazi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations

Abstract

Oxygen plays a major role in cancer biology and tumor progression. In PDT, the reduction in efficacy is directly related to lack of oxygen because its molecular mechanism relies on oxygen as an energy mediator. Measuring tumor oxygenation can provide physicians with better diagnosis and optimization of treatment plans. However, clinical tools for directly assessing tissue oxygenation are limited. The gold standard is oxygen needle electrode, which is invasive and measures oxygen level at a single location. We present our work on developing a combined treatment-imaging modality that integrates PDT and photoacoustic oxygen imaging. We propose a system designed for clinical treatments of cancer of the oral cavity. Tissue oxygen imaging is performed by applying Photoacoustic Lifetime Imaging (PALI). This technology relies on photoacoustic probing of oxygen-dependent excitation lifetime of Methylene Blue. The dye is excited by the same wavelength of illumination source for PDT. Once excited, the population of photosensitizer molecules at triplet state has a lifetime depending on the oxygen level. The transition from excited triplet state to ground state can be probe by another laser, which generate photoacoustic signal that is used to map the lifetime. The lifetime map is then converted to pO2 distribution. We expect that PDT efficacy can be improved by applying PALI imaging feedback in real-time to determine, and individually optimize, O2-enriched gas breathing parameters and PDT light-dose during treatment. Successful implementation of PALI in PDT can also drive its application in guiding other cancer treatments that are affected by hypoxia.

Original languageEnglish (US)
Title of host publicationOptical Methods for Tumor Treatment and Detection
Subtitle of host publicationMechanisms and Techniques in Photodynamic Therapy XXIII
PublisherSPIE
ISBN (Print)9780819498441
DOIs
StatePublished - Jan 1 2014
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIII - San Francisco, CA, United States
Duration: Feb 1 2014Feb 3 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8931
ISSN (Print)1605-7422

Conference

ConferenceOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIII
CountryUnited States
CitySan Francisco, CA
Period2/1/142/3/14

Keywords

  • methylene blue
  • photoacoustic imaging
  • photoacoustic lifetime imaging
  • photodynamic therapy
  • tissue oxygen

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