Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion

Reinier J. de Vries, Stephanie E.J. Cronin, Padraic Romfh, Casie A. Pendexter, Rohil Jain, Benjamin T. Wilks, Siavash Raigani, Thomas M. van Gulik, Peili Chen, Heidi Yeh, Korkut Uygun, Shannon N. Tessier

Research output: Contribution to journalArticlepeer-review

Abstract

Ischemia reperfusion injury (IRI) is a critical problem in liver transplantation that can lead to life-threatening complications and substantially limit the utilization of livers for transplantation. However, because there are no early diagnostics available, fulminant injury may only become evident post-transplant. Mitochondria play a central role in IRI and are an ideal diagnostic target. During ischemia, changes in the mitochondrial redox state form the first link in the chain of events that lead to IRI. In this study we used resonance Raman spectroscopy to provide a rapid, non-invasive, and label-free diagnostic for quantification of the hepatic mitochondrial redox status. We show this diagnostic can be used to significantly distinguish transplantable versus non-transplantable ischemically injured rat livers during oxygenated machine perfusion and demonstrate spatial differences in the response of mitochondrial redox to ischemia reperfusion. This novel diagnostic may be used in the future to predict the viability of human livers for transplantation and as a tool to better understand the mechanisms of hepatic IRI.

Original languageEnglish (US)
Article numbere0258833
JournalPloS one
Volume16
Issue number10 October
DOIs
StatePublished - Oct 2021

Bibliographical note

Publisher Copyright:
© 2021 de Vries et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

EEC 1941543

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

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