Non-invasive monitoring of stromal biophysics with targeted depletion of hyaluronan in pancreatic ductal adenocarcinoma

Ezekiel Maloney, Christopher C. Dufort, Paolo Provenzano, Navid Farr, Markus A. Carlson, Ravneet Vohra, Joshua Park, Sunil R. Hingorani, Donghoon Lee

Research output: Contribution to journalArticle

Abstract

Pancreatic ductal adenocarcinoma (PDA) is characterized by a pronounced fibroinflammatory stromal reaction consisting of inordinate levels of hyaluronan (HA), collagen, immune cells, and activated fibroblasts that work in concert to generate a robust physical barrier to the perfusion and diffusion of smallmolecule therapeutics. The targeted depletion of hyaluronanwith a PEGylated recombinant human hyaluronidase (PEGPH20) lowers interstitial gel–fluid pressures and re-expands collapsed intratumoral vasculature, improving the delivery of concurrently administered agents. Here we report a non-invasive means of assessing biophysical responses to stromal intervention with quantitative multiparametric magnetic resonance imaging (MRI) at 14 Tesla (T).We found that spin-spin relaxation time T2 values and glycosaminoglycan chemical exchange saturation transfer (GagCEST) values decreased at 24 h, reflecting depletion of intratumoral HA content, and that these parameters recovered at 7 days concurrent with replenishment of intratumoral HA. This was also reflected in an increase in low-b apparent diffusion coefficient (ADC) at 24 h, consistent with improved tumor perfusion that again normalized at 7 days after treatment. Phantom imaging suggests that the GagCEST signal is driven by changes in HA versus other glycosaminoglycans. Thus, multiparametric magnetic resonance imaging (MRI) can be used as a non-invasive tool to assess therapeutic responses to targeted stromal therapy in PDA and likely other stroma-rich solid tumors that have high levels of hyaluronan and collagen.

Original languageEnglish (US)
Article number772
JournalCancers
Volume11
Issue number6
DOIs
StatePublished - Jun 1 2019

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Biophysics
Hyaluronic Acid
Adenocarcinoma
Glycosaminoglycans
Collagen
Imaging Phantoms
Perfusion
Magnetic Resonance Imaging
Architectural Accessibility
Hyaluronoglucosaminidase
Neoplasms
Therapeutics
Fibroblasts
Pressure

Keywords

  • Hyaluronan
  • Magnetic resonance imaging
  • PEGPH20
  • Pancreatic ductal adenocarcinoma

PubMed: MeSH publication types

  • Journal Article

Cite this

Non-invasive monitoring of stromal biophysics with targeted depletion of hyaluronan in pancreatic ductal adenocarcinoma. / Maloney, Ezekiel; Dufort, Christopher C.; Provenzano, Paolo; Farr, Navid; Carlson, Markus A.; Vohra, Ravneet; Park, Joshua; Hingorani, Sunil R.; Lee, Donghoon.

In: Cancers, Vol. 11, No. 6, 772, 01.06.2019.

Research output: Contribution to journalArticle

Maloney, E, Dufort, CC, Provenzano, P, Farr, N, Carlson, MA, Vohra, R, Park, J, Hingorani, SR & Lee, D 2019, 'Non-invasive monitoring of stromal biophysics with targeted depletion of hyaluronan in pancreatic ductal adenocarcinoma', Cancers, vol. 11, no. 6, 772. https://doi.org/10.3390/cancers11060772
Maloney, Ezekiel ; Dufort, Christopher C. ; Provenzano, Paolo ; Farr, Navid ; Carlson, Markus A. ; Vohra, Ravneet ; Park, Joshua ; Hingorani, Sunil R. ; Lee, Donghoon. / Non-invasive monitoring of stromal biophysics with targeted depletion of hyaluronan in pancreatic ductal adenocarcinoma. In: Cancers. 2019 ; Vol. 11, No. 6.
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