Non-invasive bat species identification from mixed-species samples using a microarray

Lorelei E. Patrick, Jan M. Just, Maarten J. Vonhof

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Identifying species from non-invasively collected, mixed species samples for biodiversity monitoring can be difficult and expensive using typical molecular methods because samples are often degraded. This is the case when identifying the species of bats present in roosts where guano may be the only means of assessment without disturbing the bats themselves. To aid in such studies, we developed species-specific DNA probes and a microarray capable of identifying most bat species in the United States and Canada using our existing database of 16S mitochondrial DNA sequences. The microarray was sensitive enough to detect DNA diluted 1:500 for several species combinations and was able to detect the presence of more species in mixed guano samples collected from roosts than did direct sequencing from individual fecal pellets. We suggest that these DNA probes and the microarray could be a valuable tool with which to non-invasively monitor bat populations and roost use.

Original languageEnglish (US)
Pages (from-to)139-149
Number of pages11
JournalConservation Genetics Resources
Volume9
Issue number1
DOIs
StatePublished - Mar 1 2017

Fingerprint

bat
Chiroptera
DNA probes
DNA Probes
animal manures
guano
sampling
monitoring
Biodiversity
DNA
Oligonucleotide Array Sequence Analysis
Mitochondrial DNA
Canada
pellets
probe
mitochondrial DNA
Databases
biodiversity
fecal pellet
nucleotide sequences

Keywords

  • Bat
  • Degraded DNA
  • Guano
  • Microarray
  • Mixed species samples
  • Non-invasive sampling
  • Species identification

Cite this

Non-invasive bat species identification from mixed-species samples using a microarray. / Patrick, Lorelei E.; Just, Jan M.; Vonhof, Maarten J.

In: Conservation Genetics Resources, Vol. 9, No. 1, 01.03.2017, p. 139-149.

Research output: Contribution to journalArticle

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