Non-homologous sex chromosomes in two geckos (Gekkonidae: Gekkota) with female heterogamety

Kazumi Matsubara, Tony Gamble, Yoichi Matsuda, David Zarkower, Stephen D. Sarre, Arthur Georges, Jennifer A Marshall Graves, Tariq Ezaz

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Evaluating homology between the sex chromosomes of different species is an important first step in deducing the origins and evolution of sex-determining mechanisms in a clade. Here, we describe the preparation of Z and W chromosome paints via chromosome microdissection from the Australian marbled gecko (Christinus marmoratus) and their subsequent use in evaluating sex chromosome homology with the ZW chromosomes of the Kwangsi gecko (Gekko hokouensis) from eastern Asia. We show that the ZW sex chromosomes of C. marmoratus and G. hokouensis are not homologous and represent independent origins of female heterogamety within the Gekkonidae. We also show that the C. marmoratus Z and W chromosomes are genetically similar to each other as revealed by C-banding, comparative genomic hybridization, and the reciprocal painting of Z and W chromosome probes. This implies that sex chromosomes in C. marmoratus are at an early stage of differentiation, suggesting a recent origin.

Original languageEnglish (US)
Pages (from-to)251-258
Number of pages8
JournalCytogenetic and Genome Research
Volume143
Issue number4
DOIs
StatePublished - Nov 7 2014

Keywords

  • Chromosome paint
  • Cytogenetics
  • FISH
  • Homology
  • Reptilia

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    Matsubara, K., Gamble, T., Matsuda, Y., Zarkower, D., Sarre, S. D., Georges, A., Graves, J. A. M., & Ezaz, T. (2014). Non-homologous sex chromosomes in two geckos (Gekkonidae: Gekkota) with female heterogamety. Cytogenetic and Genome Research, 143(4), 251-258. https://doi.org/10.1159/000366172