Non-histone chromosomal proteins: Their role in the regulation of histone-gene expression

G. S. Stein, J. L. Stein, L. J. Kleinsmith, R. L. Jansing, W. D. Park, J. A. Thomson

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Abstract

Histogene-gene expression was studied during the cell cycle of continuously dividing HeLa S 3 cells and after stimulation of confluent monolyers of WI-38 human diploid fibroblasts to proliferate. The presence of histone-mRNA sequences was assayed by hybridization to a 3H-labelled single-stranded DNA complementary to histone-mRNA molecules. In HeLa S 3 cells histone-mRNA sequences were found in the nucleus and associated with polyribosomes during S-phase, but not during G 1-phase. Transcripts of S-phase chromatin contained histone-mRNA sequences, but those of G 1-phase chromatin did not. Similarly, in WI-38 cells association of histone-mRNA sequences with polyribosomes and transcription of histone-mRNA sequences from chromatin parallel DNA replication. Taken together these results suggest that the regulation of histone-gene expression resides, at least in part, at the transcriptional level. Chromatin reconstruction studies provide evidence that non-histone chromosomal proteins play a key role in activation of histone-gene transcription during the period of the cell cycle when DNA is replicated. Phosphate groups associated with the S-phase non-histone chromosomal proteins appear to be functionally involved in the control of histone-gene readout.

Original languageEnglish (US)
Pages (from-to)137-163
Number of pages27
JournalBiochemical Society Symposia
VolumeNo. 42
StatePublished - Dec 1 1977

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    Stein, G. S., Stein, J. L., Kleinsmith, L. J., Jansing, R. L., Park, W. D., & Thomson, J. A. (1977). Non-histone chromosomal proteins: Their role in the regulation of histone-gene expression. Biochemical Society Symposia, No. 42, 137-163.