Non-apoptotic fas (CD95) signaling on T cells regulates the resolution of Th2-mediated inflammation

Jesse W. Williams; Caroline M. Ferreira; Kelly M. Blaine; Crystal Rayon; Francisco Velázquez; Jiankun Tong; Marcus E. Peter; Anne I. Sperling

doi:10.3389/fimmu.2018.02521

Non-apoptotic fas (CD95) signaling on T cells regulates the resolution of Th2-mediated inflammation

Jesse W. Williams, Caroline M. Ferreira, Kelly M. Blaine, Crystal Rayon, Francisco Velázquez, Jiankun Tong, Marcus E. Peter, Anne I. Sperling

Pediatric Genetics & Metabolism

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Fas (CD95/APO-1) and its ligand (FasL/CD95L) promote the resolution of type 2 lung inflammation and eosinophilia. We previously found that Fas-deficiency on T cells, but not eosinophils, delayed resolution of inflammation. However, Fas can signal both cell death and have a positive signaling function that can actually activate cells. In this study, we investigated whether Fas-induced death or Fas-activated signaling pathways promote resolution of allergic lung inflammation. By increasing T cell survival through two Fas-independent pathways, using Bim-deficient T cells or Bcl-xL overexpressing T cells, no differences in resolution of Th2-mediated inflammation was observed. Furthermore, Th2 cells were inherently resistant to Fas-mediated apoptosis and preferentially signaled through non-apoptotic pathways following FasL treatment. Utilizing Fas-mutant mice deficient in apoptotic but sufficient for non-apoptotic Fas signaling pathways, we demonstrate that non-apoptotic Fas signaling in T cells drives resolution of Th2-mediated airway inflammation. Our findings reveal a previously unknown role for non-apoptotic Fas signaling on Th2 cells in the induction of resolution of type 2 inflammation.

Original language	English (US)
Article number	02521
Journal	Frontiers in immunology
Volume	9
Issue number	NOV
DOIs	https://doi.org/10.3389/fimmu.2018.02521
State	Published - Nov 1 2018

Bibliographical note

Publisher Copyright:
Copyright © 2018 Williams, Ferreira, Blaine, Rayon, Velázquez, Tong, Peter and Sperling. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Keywords

Allergy
Apoptosis
Asthma
Eosinophilia
Fas-FasL
Th2 cells

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3389/fimmu.2018.02521

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title = "Non-apoptotic fas (CD95) signaling on T cells regulates the resolution of Th2-mediated inflammation",

abstract = "Fas (CD95/APO-1) and its ligand (FasL/CD95L) promote the resolution of type 2 lung inflammation and eosinophilia. We previously found that Fas-deficiency on T cells, but not eosinophils, delayed resolution of inflammation. However, Fas can signal both cell death and have a positive signaling function that can actually activate cells. In this study, we investigated whether Fas-induced death or Fas-activated signaling pathways promote resolution of allergic lung inflammation. By increasing T cell survival through two Fas-independent pathways, using Bim-deficient T cells or Bcl-xL overexpressing T cells, no differences in resolution of Th2-mediated inflammation was observed. Furthermore, Th2 cells were inherently resistant to Fas-mediated apoptosis and preferentially signaled through non-apoptotic pathways following FasL treatment. Utilizing Fas-mutant mice deficient in apoptotic but sufficient for non-apoptotic Fas signaling pathways, we demonstrate that non-apoptotic Fas signaling in T cells drives resolution of Th2-mediated airway inflammation. Our findings reveal a previously unknown role for non-apoptotic Fas signaling on Th2 cells in the induction of resolution of type 2 inflammation.",

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author = "Williams, {Jesse W.} and Ferreira, {Caroline M.} and Blaine, {Kelly M.} and Crystal Rayon and Francisco Vel{\'a}zquez and Jiankun Tong and Peter, {Marcus E.} and Sperling, {Anne I.}",

note = "Publisher Copyright: Copyright {\textcopyright} 2018 Williams, Ferreira, Blaine, Rayon, Vel{\'a}zquez, Tong, Peter and Sperling. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.",

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AU - Williams, Jesse W.

AU - Ferreira, Caroline M.

AU - Blaine, Kelly M.

AU - Rayon, Crystal

AU - Velázquez, Francisco

AU - Tong, Jiankun

AU - Peter, Marcus E.

AU - Sperling, Anne I.

N1 - Publisher Copyright: Copyright © 2018 Williams, Ferreira, Blaine, Rayon, Velázquez, Tong, Peter and Sperling. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Fas (CD95/APO-1) and its ligand (FasL/CD95L) promote the resolution of type 2 lung inflammation and eosinophilia. We previously found that Fas-deficiency on T cells, but not eosinophils, delayed resolution of inflammation. However, Fas can signal both cell death and have a positive signaling function that can actually activate cells. In this study, we investigated whether Fas-induced death or Fas-activated signaling pathways promote resolution of allergic lung inflammation. By increasing T cell survival through two Fas-independent pathways, using Bim-deficient T cells or Bcl-xL overexpressing T cells, no differences in resolution of Th2-mediated inflammation was observed. Furthermore, Th2 cells were inherently resistant to Fas-mediated apoptosis and preferentially signaled through non-apoptotic pathways following FasL treatment. Utilizing Fas-mutant mice deficient in apoptotic but sufficient for non-apoptotic Fas signaling pathways, we demonstrate that non-apoptotic Fas signaling in T cells drives resolution of Th2-mediated airway inflammation. Our findings reveal a previously unknown role for non-apoptotic Fas signaling on Th2 cells in the induction of resolution of type 2 inflammation.

AB - Fas (CD95/APO-1) and its ligand (FasL/CD95L) promote the resolution of type 2 lung inflammation and eosinophilia. We previously found that Fas-deficiency on T cells, but not eosinophils, delayed resolution of inflammation. However, Fas can signal both cell death and have a positive signaling function that can actually activate cells. In this study, we investigated whether Fas-induced death or Fas-activated signaling pathways promote resolution of allergic lung inflammation. By increasing T cell survival through two Fas-independent pathways, using Bim-deficient T cells or Bcl-xL overexpressing T cells, no differences in resolution of Th2-mediated inflammation was observed. Furthermore, Th2 cells were inherently resistant to Fas-mediated apoptosis and preferentially signaled through non-apoptotic pathways following FasL treatment. Utilizing Fas-mutant mice deficient in apoptotic but sufficient for non-apoptotic Fas signaling pathways, we demonstrate that non-apoptotic Fas signaling in T cells drives resolution of Th2-mediated airway inflammation. Our findings reveal a previously unknown role for non-apoptotic Fas signaling on Th2 cells in the induction of resolution of type 2 inflammation.

KW - Allergy

KW - Apoptosis

KW - Asthma

KW - Eosinophilia

KW - Fas-FasL

KW - Th2 cells

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ER -

Non-apoptotic fas (CD95) signaling on T cells regulates the resolution of Th2-mediated inflammation

Abstract

Bibliographical note

Keywords

PubMed: MeSH publication types

UN SDGs

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