Noggin is required for normal lobe patterning and ductal budding in the mouse prostate

Crist Cook; Chad M. Vezina; Sarah H. Allgeier; Aubie Shaw; Min Yu; Richard E. Peterson; Wade Bushman

doi:10.1016/j.ydbio.2007.09.038

Noggin is required for normal lobe patterning and ductal budding in the mouse prostate

Crist Cook, Chad M. Vezina, Sarah H. Allgeier, Aubie Shaw, Min Yu, Richard E. Peterson, Wade Bushman

Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

Mesenchymal expression of the BMP antagonist NOGGIN during prostate development plays a critical role in pre-natal ventral prostate development and opposes BMP4-mediated inhibition of cell proliferation during postnatal ductal development. Morphologic examination of newborn Noggin^-/- male fetuses revealed genitourinary anomalies including cryptorchidism, incomplete separation of the hindgut from the urogenital sinus (UGS), absence of the ventral mesenchymal pad, and a complete loss of ventral prostate (VP) budding. Examination of lobe-specific marker expression in the E14 Noggin^-/- UGS rescued by transplantation under the renal capsule of a male nude mouse confirmed a complete loss of VP determination. More modest effects were observed in the other lobes, including decreased number of ductal buds in the dorsal and lateral prostates of newborn Noggin^-/- males. BMP4 and BMP7 have been shown to inhibit ductal budding and outgrowth by negatively regulating epithelial cell proliferation. We show here that NOGGIN can neutralize budding inhibition by BMP4 and rescues branching morphogenesis of BMP4-exposed UGS in organ culture and show that the effects of BMP4 and NOGGIN activities converge on P63⁺ epithelial cells located at nascent duct tips. Together, these studies show that the BMP-NOGGIN axis regulates patterning of the ventral prostate, regulates ductal budding, and controls proliferation of P63⁺ epithelial cells in the nascent ducts of developing mouse prostate.

Original language	English (US)
Pages (from-to)	217-230
Number of pages	14
Journal	Developmental Biology
Volume	312
Issue number	1
DOIs	https://doi.org/10.1016/j.ydbio.2007.09.038
State	Published - Dec 1 2007

Bibliographical note

Funding Information:
The authors would like to thank Brigid Hogan for supplying a breeder pair of Noggin tm1(Lacz)Am mice, Edward DeRobertis for supplying the Chordin knockout mice, the UW Flow Cytometry Lab for its use of the fluorescence microscope, Jerry Gipp and Rob Lipinski for their contributions to the cell regulation studies and mouse colony maintenance, and Xin Sun (UW-Madison) for providing the mouse Noggin cDNA and Gremlin knockout mice. This work was supported by the following Peterson Lab grants: NIH P50 DK065303, NIH R37 ES01332, F32 ES014284, and F31 HD049323 and Bushman Lab grants: NIH P50 DK052687, NIH O'Brien DK065303, and DOD W81XWH.

Keywords

Bmp
Development
Noggin
Prostate
Urogenital sinus

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10.1016/j.ydbio.2007.09.038

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title = "Noggin is required for normal lobe patterning and ductal budding in the mouse prostate",

abstract = "Mesenchymal expression of the BMP antagonist NOGGIN during prostate development plays a critical role in pre-natal ventral prostate development and opposes BMP4-mediated inhibition of cell proliferation during postnatal ductal development. Morphologic examination of newborn Noggin-/- male fetuses revealed genitourinary anomalies including cryptorchidism, incomplete separation of the hindgut from the urogenital sinus (UGS), absence of the ventral mesenchymal pad, and a complete loss of ventral prostate (VP) budding. Examination of lobe-specific marker expression in the E14 Noggin-/- UGS rescued by transplantation under the renal capsule of a male nude mouse confirmed a complete loss of VP determination. More modest effects were observed in the other lobes, including decreased number of ductal buds in the dorsal and lateral prostates of newborn Noggin-/- males. BMP4 and BMP7 have been shown to inhibit ductal budding and outgrowth by negatively regulating epithelial cell proliferation. We show here that NOGGIN can neutralize budding inhibition by BMP4 and rescues branching morphogenesis of BMP4-exposed UGS in organ culture and show that the effects of BMP4 and NOGGIN activities converge on P63+ epithelial cells located at nascent duct tips. Together, these studies show that the BMP-NOGGIN axis regulates patterning of the ventral prostate, regulates ductal budding, and controls proliferation of P63+ epithelial cells in the nascent ducts of developing mouse prostate.",

keywords = "Bmp, Development, Noggin, Prostate, Urogenital sinus",

author = "Crist Cook and Vezina, {Chad M.} and Allgeier, {Sarah H.} and Aubie Shaw and Min Yu and Peterson, {Richard E.} and Wade Bushman",

note = "Funding Information: The authors would like to thank Brigid Hogan for supplying a breeder pair of Noggin tm1(Lacz)Am mice, Edward DeRobertis for supplying the Chordin knockout mice, the UW Flow Cytometry Lab for its use of the fluorescence microscope, Jerry Gipp and Rob Lipinski for their contributions to the cell regulation studies and mouse colony maintenance, and Xin Sun (UW-Madison) for providing the mouse Noggin cDNA and Gremlin knockout mice. This work was supported by the following Peterson Lab grants: NIH P50 DK065303, NIH R37 ES01332, F32 ES014284, and F31 HD049323 and Bushman Lab grants: NIH P50 DK052687, NIH O'Brien DK065303, and DOD W81XWH. ",

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AU - Allgeier, Sarah H.

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AU - Yu, Min

AU - Peterson, Richard E.

AU - Bushman, Wade

N1 - Funding Information: The authors would like to thank Brigid Hogan for supplying a breeder pair of Noggin tm1(Lacz)Am mice, Edward DeRobertis for supplying the Chordin knockout mice, the UW Flow Cytometry Lab for its use of the fluorescence microscope, Jerry Gipp and Rob Lipinski for their contributions to the cell regulation studies and mouse colony maintenance, and Xin Sun (UW-Madison) for providing the mouse Noggin cDNA and Gremlin knockout mice. This work was supported by the following Peterson Lab grants: NIH P50 DK065303, NIH R37 ES01332, F32 ES014284, and F31 HD049323 and Bushman Lab grants: NIH P50 DK052687, NIH O'Brien DK065303, and DOD W81XWH.

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N2 - Mesenchymal expression of the BMP antagonist NOGGIN during prostate development plays a critical role in pre-natal ventral prostate development and opposes BMP4-mediated inhibition of cell proliferation during postnatal ductal development. Morphologic examination of newborn Noggin-/- male fetuses revealed genitourinary anomalies including cryptorchidism, incomplete separation of the hindgut from the urogenital sinus (UGS), absence of the ventral mesenchymal pad, and a complete loss of ventral prostate (VP) budding. Examination of lobe-specific marker expression in the E14 Noggin-/- UGS rescued by transplantation under the renal capsule of a male nude mouse confirmed a complete loss of VP determination. More modest effects were observed in the other lobes, including decreased number of ductal buds in the dorsal and lateral prostates of newborn Noggin-/- males. BMP4 and BMP7 have been shown to inhibit ductal budding and outgrowth by negatively regulating epithelial cell proliferation. We show here that NOGGIN can neutralize budding inhibition by BMP4 and rescues branching morphogenesis of BMP4-exposed UGS in organ culture and show that the effects of BMP4 and NOGGIN activities converge on P63+ epithelial cells located at nascent duct tips. Together, these studies show that the BMP-NOGGIN axis regulates patterning of the ventral prostate, regulates ductal budding, and controls proliferation of P63+ epithelial cells in the nascent ducts of developing mouse prostate.

AB - Mesenchymal expression of the BMP antagonist NOGGIN during prostate development plays a critical role in pre-natal ventral prostate development and opposes BMP4-mediated inhibition of cell proliferation during postnatal ductal development. Morphologic examination of newborn Noggin-/- male fetuses revealed genitourinary anomalies including cryptorchidism, incomplete separation of the hindgut from the urogenital sinus (UGS), absence of the ventral mesenchymal pad, and a complete loss of ventral prostate (VP) budding. Examination of lobe-specific marker expression in the E14 Noggin-/- UGS rescued by transplantation under the renal capsule of a male nude mouse confirmed a complete loss of VP determination. More modest effects were observed in the other lobes, including decreased number of ductal buds in the dorsal and lateral prostates of newborn Noggin-/- males. BMP4 and BMP7 have been shown to inhibit ductal budding and outgrowth by negatively regulating epithelial cell proliferation. We show here that NOGGIN can neutralize budding inhibition by BMP4 and rescues branching morphogenesis of BMP4-exposed UGS in organ culture and show that the effects of BMP4 and NOGGIN activities converge on P63+ epithelial cells located at nascent duct tips. Together, these studies show that the BMP-NOGGIN axis regulates patterning of the ventral prostate, regulates ductal budding, and controls proliferation of P63+ epithelial cells in the nascent ducts of developing mouse prostate.

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KW - Urogenital sinus

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Noggin is required for normal lobe patterning and ductal budding in the mouse prostate

Abstract

Bibliographical note

Keywords

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