Nodule-specific PLAT domain proteins are expanded in the Medicago lineage and required for nodulation

Research output: Contribution to journalArticle

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Abstract

Symbiotic nitrogen fixation in legumes is mediated by an interplay of signaling processes between plant hosts and rhizobial symbionts. In legumes, several secreted protein families have undergone expansions and play key roles in nodulation. Thus, identifying lineage-specific expansions (LSEs) of nodulation-associated genes can be a strategy to discover candidate gene families. Using bioinformatic tools, we identified 13 LSEs of nodulation-related secreted protein families, each unique to either Glycine, Arachis or Medicago lineages. In the Medicago lineage, nodule-specific Polycystin-1, Lipoxygenase, Alpha Toxin (PLAT) domain proteins (NPDs) expanded to five members. We examined NPD function using CRISPR/Cas9 multiplex genome editing to create Medicago truncatula NPD knockout lines, targeting one to five NPD genes. Mutant lines with differing combinations of NPD gene inactivations had progressively smaller nodules, earlier onset of nodule senescence, or ineffective nodules compared to the wild-type control. Double- and triple-knockout lines showed dissimilar nodulation phenotypes but coincided in upregulation of a DHHC-type zinc finger and an aspartyl protease gene, possible candidates for the observed disturbance of proper nodule function. By postulating that gene family expansions can be used to detect candidate genes, we identified a family of nodule-specific PLAT domain proteins and confirmed that they play a role in successful nodule formation.

Original languageEnglish (US)
Pages (from-to)1538-1550
Number of pages13
JournalNew Phytologist
Volume222
Issue number3
DOIs
StatePublished - May 1 2019

Fingerprint

Medicago
Lipoxygenase
lipoxygenase
nodulation
toxins
Genes
genes
proteins
Fabaceae
Clustered Regularly Interspaced Short Palindromic Repeats
Medicago truncatula
Aspartic Acid Proteases
legumes
Nitrogen Fixation
Glycine (Fabaceae)
aspartic proteinases
Zinc Fingers
Arachis
Gene Silencing
Computational Biology

Keywords

  • lineage-specific expansion
  • multiplex genome editing
  • nodulation-related secreted proteins
  • nodule organogenesis
  • nodule-specific PLAT domain

Cite this

Nodule-specific PLAT domain proteins are expanded in the Medicago lineage and required for nodulation. / Trujillo, Diana I; Silverstein, Kevin A; Young, Nevin D.

In: New Phytologist, Vol. 222, No. 3, 01.05.2019, p. 1538-1550.

Research output: Contribution to journalArticle

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