NMR-spectroscopic screening of spider venom reveals sulfated nucleosides as major components for the brown recluse and related species

Frank C. Schroeder, Andrew E. Taggi, Matthew Gronquist, Rabia U. Malik, Jacqualine B. Grant, Thomas Eisner, Jerrold Meinwald

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Extensive chemical analyses of spider venoms from many species have revealed complex mixtures of biologically active compounds, of which several have provided important leads for drug development. We have recently shown that NMR spectroscopy can be used advantageously for a direct structural characterization of the small-molecule content of such complex mixtures. Here, we report the application of this strategy to a larger-scale analysis of a collection of spider venoms representing >70 species, which, in combination with mass spectrometric analyses, allowed the identification of a wide range of known, and several previously undescribed, small molecules. These include polyamines, common neurotransmitters, and amino acid derivatives as well as two additional members of a recently discovered family of natural products, the sulfated nucleosides. In the case of the well studied brown recluse spider, Loxosceles reclusa, sulfated guanosine derivatives were found to comprise the major small-molecule components of the venom.

Original languageEnglish (US)
Pages (from-to)14283-14287
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number38
DOIs
StatePublished - Sep 23 2008

Keywords

  • Chemical prospecting
  • Loxosceles
  • Metabolomics
  • Natural products
  • Neurotoxins

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