NMR Characterization of Polyethylene Glycol Conjugates for Nanoparticle Functionalization

Jacqueline L. Pasek-Allen, Randall K. Wilharm, John C. Bischof, Valérie C. Pierre

Research output: Contribution to journalArticlepeer-review


The molecular weight, purity, and functionalization of polyethylene glycols are often characterized by 1H NMR spectroscopy. Oft-forgotten, the typical 1H NMR pulse sequence is not 13C decoupled. Hence, for large polymers, the 13C coupled 1H peaks arising from the repeating units have integrations comparable to that of the 1H of the terminal groups. Ignoring this coupling leads to erroneous assignments. Once correctly assigned, these 13C coupled 1H peaks can be used to determine both the molecular weight of the polymer and the efficacy of conjugation of a terminal moiety more accurately than the uncoupled 1H of the repeating unit.

Original languageEnglish (US)
JournalACS Omega
StateAccepted/In press - 2022

Bibliographical note

Funding Information:
The authors thank Dr. Peter Flynn at the University of Utah, and Dr. Todd Rappe and Dr. Letitia Yao at the University of Minnesota for useful discussions. This work was supported by 5 F31 DK124968-02, NSF EEC 1941543, R01 HL135046, DK124333, and R01 DK117425. Research reported in this publication was supported by the Office of the Vice President of Research, College of Science and Engineering at the University of Minnesota.

Publisher Copyright:
© 2023 The Authors. Published by American Chemical Society.

PubMed: MeSH publication types

  • Journal Article


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