NMDA receptor mRNA expression in NOS-containing neurons in the spinal trigeminal nucleus of the rat

C. S. Dohrn; Al J Beitz

doi:10.1016/0304-3940(94)91070-7

NMDA receptor mRNA expression in NOS-containing neurons in the spinal trigeminal nucleus of the rat

C. S. Dohrn, Al J Beitz

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23 Scopus citations

Abstract

The spinal trigeminal nucleus (STN) is involved in the transmission of orofacial sensory information. Nitric oxide (NO), an important neuromessenger, and the glutamate receptor subtype, NMDA NR1, have been implicated in nociception in the STN. However, the anatomical relationship of NO and NMDA NR1 has not been investigated within this nucleus. Using both immunocytochemical (against NO synthase; NOS) and in situ hybridization studies of NMDA NR1 receptor mRNA, we found that NOS-containing neurons in the STN expressed more mRNA for NR1 than did non-NOS-containing neurons in the STN. These data suggest that NMDA activation may lead to NO production in the STN and is consistent with previous studies, implicating both NMDA and NO in nociception.

Original language	English (US)
Pages (from-to)	28-32
Number of pages	5
Journal	Neuroscience Letters
Volume	175
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(94)91070-7
State	Published - Jul 4 1994

Bibliographical note

Funding Information:
This study has been supported by NIDR DE06682. Special thanks to Mary Mullett, Lori Wellman, Richard H. Price and Jennifer J. Sanderson for their technical assistance and advice.

Keywords

Brainstem
Glutamate
Nitric oxide
Trigeminal nucleus

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10.1016/0304-3940(94)91070-7

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title = "NMDA receptor mRNA expression in NOS-containing neurons in the spinal trigeminal nucleus of the rat",

abstract = "The spinal trigeminal nucleus (STN) is involved in the transmission of orofacial sensory information. Nitric oxide (NO), an important neuromessenger, and the glutamate receptor subtype, NMDA NR1, have been implicated in nociception in the STN. However, the anatomical relationship of NO and NMDA NR1 has not been investigated within this nucleus. Using both immunocytochemical (against NO synthase; NOS) and in situ hybridization studies of NMDA NR1 receptor mRNA, we found that NOS-containing neurons in the STN expressed more mRNA for NR1 than did non-NOS-containing neurons in the STN. These data suggest that NMDA activation may lead to NO production in the STN and is consistent with previous studies, implicating both NMDA and NO in nociception.",

keywords = "Brainstem, Glutamate, Nitric oxide, Trigeminal nucleus",

author = "Dohrn, {C. S.} and Beitz, {Al J}",

note = "Funding Information: This study has been supported by NIDR DE06682. Special thanks to Mary Mullett, Lori Wellman, Richard H. Price and Jennifer J. Sanderson for their technical assistance and advice.",

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T1 - NMDA receptor mRNA expression in NOS-containing neurons in the spinal trigeminal nucleus of the rat

AU - Dohrn, C. S.

AU - Beitz, Al J

N1 - Funding Information: This study has been supported by NIDR DE06682. Special thanks to Mary Mullett, Lori Wellman, Richard H. Price and Jennifer J. Sanderson for their technical assistance and advice.

PY - 1994/7/4

Y1 - 1994/7/4

N2 - The spinal trigeminal nucleus (STN) is involved in the transmission of orofacial sensory information. Nitric oxide (NO), an important neuromessenger, and the glutamate receptor subtype, NMDA NR1, have been implicated in nociception in the STN. However, the anatomical relationship of NO and NMDA NR1 has not been investigated within this nucleus. Using both immunocytochemical (against NO synthase; NOS) and in situ hybridization studies of NMDA NR1 receptor mRNA, we found that NOS-containing neurons in the STN expressed more mRNA for NR1 than did non-NOS-containing neurons in the STN. These data suggest that NMDA activation may lead to NO production in the STN and is consistent with previous studies, implicating both NMDA and NO in nociception.

AB - The spinal trigeminal nucleus (STN) is involved in the transmission of orofacial sensory information. Nitric oxide (NO), an important neuromessenger, and the glutamate receptor subtype, NMDA NR1, have been implicated in nociception in the STN. However, the anatomical relationship of NO and NMDA NR1 has not been investigated within this nucleus. Using both immunocytochemical (against NO synthase; NOS) and in situ hybridization studies of NMDA NR1 receptor mRNA, we found that NOS-containing neurons in the STN expressed more mRNA for NR1 than did non-NOS-containing neurons in the STN. These data suggest that NMDA activation may lead to NO production in the STN and is consistent with previous studies, implicating both NMDA and NO in nociception.

KW - Brainstem

KW - Glutamate

KW - Nitric oxide

KW - Trigeminal nucleus

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JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

NMDA receptor mRNA expression in NOS-containing neurons in the spinal trigeminal nucleus of the rat

Abstract

Bibliographical note

Keywords

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