Nitrotyrosine attenuates the hemodynamic effects of adrenoceptor agonists in vivo: Relevance to the pathophysiology of peroxynitrite

Neil W. Kooy, Stephen J. Lewis

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Peroxynitrite, which attenuates catecholamine-mediated hemodynamic responses in vivo, nitrates free tyrosine residues to form the specific product, 3-nitro-L-tyrosine. The chemical structure of 3-nitro-L-tyrosine is similar to that of the endogenous catecholamines. Therefore, 3-nitro-L-tyrosine may interfere with catecholamine hemodynamic function in vivo. The hemodynamic responses produced by norepinephrine (1-4 μg/kg, i.v., n = 6), epinephrine (0.5-4 μg/kg, i.v., n = 7), phenylephrine (1-8 μg/kg, i.v., n = 5), and isoproterenol (100-400 ng/kg, i.v., n = 5) were attenuated, while the hemodynamic responses produced by arginine vasopressin (50-250 ng/kg; i.v., n = 5) were unaffected following the administration of 3-nitro-L-tyrosine (2.5 μmol/kg, i.v.) in pentobarbital-anesthetized rats. These results demonstrate substantial and selective attenuation of the hemodynamic effects produced by α- and β-adrenoceptor agonists, raising the possibility that 3-nitro-L-tyrosine may play a role in the hemodynamic dysfunction associated with inflammatory conditions in which the formation of peroxynitrite is favored.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalEuropean Journal of Pharmacology
Volume310
Issue number2-3
DOIs
StatePublished - Aug 29 1996

Keywords

  • Hemodynamics, in vivo
  • Nitric oxide (NO)
  • Nitrotyrosine
  • Peroxynitrite
  • Superoxide

Fingerprint

Dive into the research topics of 'Nitrotyrosine attenuates the hemodynamic effects of adrenoceptor agonists in vivo: Relevance to the pathophysiology of peroxynitrite'. Together they form a unique fingerprint.

Cite this