Nitrogen deficiency in maize fields of the Southern Pampas does not affect kernel number but reduces weight per kernel.

Nitrogen (N) requirement of maize has been increasing over the past decades, but there has been a lack of adjustment in fertilization management practices across different regions in Argentina. The higher N requirement in modern hybrids is associated with a higher post-flowering N accumulation. Additionally, soil fertility in the Argentina Pampas has consistently decreased in recent years. Whether the effects of N deficiency are expressed in the post-flowering period is still unclear at field level. The objective of this work was to quantify the yield reduction attributed to N deficiency and to identify the physiological basis of this effect at the field level for typical high-tech farms across the south-eastern Pampas of Argentina. Nineteen experiments were conducted during the 2020/2021 growing season in the south-eastern Pampas, evaluating two N managements i) Farmers' N level; ii) High N input. Results showed that the additional N input increased grain yield by an average of 6.4% (average grain yield difference of 712 kg ha⁻¹). Weight per kernel was the only yield component that responded to N fertilization. Farmers' N management did not affect N accumulation or growth-related variables such as leaf area index, green leaves per plant, PAR interception, nitrogen sufficiency index until flowering, but the farmers' N level reduced these variables during the grain filling period leading to differences in aerial biomass and N accumulated at physiological maturity. Farmers' N level led to decreased post-flowering N uptake during grain filling period, which reduced the photosynthetic capacity of the canopy. These findings suggest that N management strategies and models need to be reviewed for high-tech maize systems.