Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity: Possible involvement of cyclic GMP

Tobias Polte; Stefanie Oberle; Henning Schröder

doi:10.1016/S0014-5793(97)00480-8

Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity: Possible involvement of cyclic GMP

Tobias Polte
, Stefanie Oberle
, Henning Schröder

Pharmaceutics

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

In cultured endothelial cells, incubation with TNF-α (50 ng/ml) for 48 h markedly reduced viability of endothelial cells. A 6 h preincubation with Sper/NO (0.03- -1 μM) protected endothelial cells in a concentration-dependent manner and increased viability by 63% of control. The NO scavenger PTIO (30 μM) completely abolished cytoprotection by Sper/NO. A cytoprotective effect comparable to Sper/NO was observed when preincubating the cells with 8-bromo cyclic GMP (1-10 μM). Moreover, no protection by Sper/NO occurred in the presence of ODQ (0.1 μM), a selective inhibitor of soluble guanylyl cyclase. Our results demonstrate that NO produces a long-term endothelial protection against cellular injury by TNF-α, presumably via a cyclic GMP-dependent pathway.

Original language	English (US)
Pages (from-to)	46-48
Number of pages	3
Journal	FEBS Letters
Volume	409
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(97)00480-8
State	Published - Jun 2 1997

Bibliographical note

Funding Information:
This work was supported by the Deutsche Forschungsgemeinschaft (Schr 298/8-1). We would like to thank Martina Heidler for assistance in the preparation of this manuscript.

Keywords

Cyclic GMP
Cytoprotection
Endothelial cell
Nitric oxide
Tumor necrosis factor-α

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10.1016/S0014-5793(97)00480-8

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@article{7394121e5c3049d394b65dbfeb5ce65d,

title = "Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity: Possible involvement of cyclic GMP",

abstract = "In cultured endothelial cells, incubation with TNF-α (50 ng/ml) for 48 h markedly reduced viability of endothelial cells. A 6 h preincubation with Sper/NO (0.03- -1 μM) protected endothelial cells in a concentration-dependent manner and increased viability by 63\% of control. The NO scavenger PTIO (30 μM) completely abolished cytoprotection by Sper/NO. A cytoprotective effect comparable to Sper/NO was observed when preincubating the cells with 8-bromo cyclic GMP (1-10 μM). Moreover, no protection by Sper/NO occurred in the presence of ODQ (0.1 μM), a selective inhibitor of soluble guanylyl cyclase. Our results demonstrate that NO produces a long-term endothelial protection against cellular injury by TNF-α, presumably via a cyclic GMP-dependent pathway.",

keywords = "Cyclic GMP, Cytoprotection, Endothelial cell, Nitric oxide, Tumor necrosis factor-α",

author = "Tobias Polte and Stefanie Oberle and Henning Schr{\"o}der",

note = "Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft (Schr 298/8-1). We would like to thank Martina Heidler for assistance in the preparation of this manuscript.",

year = "1997",

month = jun,

day = "2",

doi = "10.1016/S0014-5793(97)00480-8",

language = "English (US)",

volume = "409",

pages = "46--48",

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TY - JOUR

T1 - Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity

T2 - Possible involvement of cyclic GMP

AU - Polte, Tobias

AU - Oberle, Stefanie

AU - Schröder, Henning

N1 - Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft (Schr 298/8-1). We would like to thank Martina Heidler for assistance in the preparation of this manuscript.

PY - 1997/6/2

Y1 - 1997/6/2

N2 - In cultured endothelial cells, incubation with TNF-α (50 ng/ml) for 48 h markedly reduced viability of endothelial cells. A 6 h preincubation with Sper/NO (0.03- -1 μM) protected endothelial cells in a concentration-dependent manner and increased viability by 63% of control. The NO scavenger PTIO (30 μM) completely abolished cytoprotection by Sper/NO. A cytoprotective effect comparable to Sper/NO was observed when preincubating the cells with 8-bromo cyclic GMP (1-10 μM). Moreover, no protection by Sper/NO occurred in the presence of ODQ (0.1 μM), a selective inhibitor of soluble guanylyl cyclase. Our results demonstrate that NO produces a long-term endothelial protection against cellular injury by TNF-α, presumably via a cyclic GMP-dependent pathway.

AB - In cultured endothelial cells, incubation with TNF-α (50 ng/ml) for 48 h markedly reduced viability of endothelial cells. A 6 h preincubation with Sper/NO (0.03- -1 μM) protected endothelial cells in a concentration-dependent manner and increased viability by 63% of control. The NO scavenger PTIO (30 μM) completely abolished cytoprotection by Sper/NO. A cytoprotective effect comparable to Sper/NO was observed when preincubating the cells with 8-bromo cyclic GMP (1-10 μM). Moreover, no protection by Sper/NO occurred in the presence of ODQ (0.1 μM), a selective inhibitor of soluble guanylyl cyclase. Our results demonstrate that NO produces a long-term endothelial protection against cellular injury by TNF-α, presumably via a cyclic GMP-dependent pathway.

KW - Cyclic GMP

KW - Cytoprotection

KW - Endothelial cell

KW - Nitric oxide

KW - Tumor necrosis factor-α

UR - https://www.scopus.com/pages/publications/0030927423

UR - https://www.scopus.com/pages/publications/0030927423#tab=citedBy

U2 - 10.1016/S0014-5793(97)00480-8

DO - 10.1016/S0014-5793(97)00480-8

M3 - Article

C2 - 9199501

AN - SCOPUS:0030927423

SN - 0014-5793

VL - 409

SP - 46

EP - 48

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity: Possible involvement of cyclic GMP

Abstract

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