Nitric oxide protects endothelial cells from tumor necrosis factor-α-mediated cytotoxicity: Possible involvement of cyclic GMP

Tobias Polte, Stefanie Oberle, Henning Schröder

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57 Scopus citations

Abstract

In cultured endothelial cells, incubation with TNF-α (50 ng/ml) for 48 h markedly reduced viability of endothelial cells. A 6 h preincubation with Sper/NO (0.03- -1 μM) protected endothelial cells in a concentration-dependent manner and increased viability by 63% of control. The NO scavenger PTIO (30 μM) completely abolished cytoprotection by Sper/NO. A cytoprotective effect comparable to Sper/NO was observed when preincubating the cells with 8-bromo cyclic GMP (1-10 μM). Moreover, no protection by Sper/NO occurred in the presence of ODQ (0.1 μM), a selective inhibitor of soluble guanylyl cyclase. Our results demonstrate that NO produces a long-term endothelial protection against cellular injury by TNF-α, presumably via a cyclic GMP-dependent pathway.

Original languageEnglish (US)
Pages (from-to)46-48
Number of pages3
JournalFEBS Letters
Volume409
Issue number1
DOIs
StatePublished - Jun 2 1997

Bibliographical note

Funding Information:
This work was supported by the Deutsche Forschungsgemeinschaft (Schr 298/8-1). We would like to thank Martina Heidler for assistance in the preparation of this manuscript.

Keywords

  • Cyclic GMP
  • Cytoprotection
  • Endothelial cell
  • Nitric oxide
  • Tumor necrosis factor-α

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