TY - JOUR
T1 - Nitric oxide production and neurotoxicity mediated by activated microglia from human versus mouse brain
AU - Peterson, Phillip K.
AU - Hu, Shuxian
AU - Robert Anderson, W.
AU - Chao, Chun C.
PY - 1994/8
Y1 - 1994/8
N2 - Recent studies indicate that human macrophages lack a high-output inducible nitric oxide synthase (NOS) antimicrobial system, In the present study, microglial cells derived from fetal human versus neonatal mouse brain were compared in a coculture assay of human and murine neuronal cell injury, Neurotoxicity (reflected by lactate dehydrogenase release and impaired neuronal uptake of [3H)γ-amino butyric acid) and nitric oxide (NO) production (assessed by measurement of nitrite) were observed only in cocultures containing interferon (IFN)-γ-lipopolysaccharide (LPS)-stimulated murine microglia. Cultures of purified human fetal microglia, however, did produce low levels of NO upon stimulation with IFN-γ-LPS, These findings support the proposal that human macrophages have an inefficient IFN-γ-inducible NOS and suggest that in tissues, such as brain, this deficiency could be advantageous for neighboring cells.
AB - Recent studies indicate that human macrophages lack a high-output inducible nitric oxide synthase (NOS) antimicrobial system, In the present study, microglial cells derived from fetal human versus neonatal mouse brain were compared in a coculture assay of human and murine neuronal cell injury, Neurotoxicity (reflected by lactate dehydrogenase release and impaired neuronal uptake of [3H)γ-amino butyric acid) and nitric oxide (NO) production (assessed by measurement of nitrite) were observed only in cocultures containing interferon (IFN)-γ-lipopolysaccharide (LPS)-stimulated murine microglia. Cultures of purified human fetal microglia, however, did produce low levels of NO upon stimulation with IFN-γ-LPS, These findings support the proposal that human macrophages have an inefficient IFN-γ-inducible NOS and suggest that in tissues, such as brain, this deficiency could be advantageous for neighboring cells.
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U2 - 10.1093/infdis/170.2.457
DO - 10.1093/infdis/170.2.457
M3 - Article
C2 - 8035037
AN - SCOPUS:0028342494
VL - 170
SP - 457
EP - 460
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 2
ER -