Nitric Oxide Methods in Seed Biology

Paul C. Bethke, Igor G L Libourel, Jan Vitecek, Russell L. Jones

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations


The ubiquitous signaling molecule nitric oxide (NO) plays an important role in seed biology. Experiments with this biologically important gas require special provisions because NO in aerobic environments is readily converted into other oxides of nitrogen. In this chapter, we describe methods for the application of NO as a gas, and through the use of NO-donor compounds. We included information on the removal or reduction of NO with NO scavengers. Methods for detecting NO using NO-reactive fluorescent probes, and an apparatus incorporating an oxidizer column are also described.

Original languageEnglish (US)
Title of host publicationSeed Dormancy
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages16
ISBN (Print)9781617792304
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© 2011, Springer Science+Business Media, LLC.


  • Application of nitric oxide
  • Nitrate and nitrite
  • Nitric oxide detection
  • Nitric oxide donors and scavengers
  • Oxidation of nitric oxide
  • Seed dormancy and germination


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