TY - JOUR
T1 - Nitric oxide inhibits ACh-induced intracellular calcium oscillations in porcine tracheal smooth muscle
AU - Prakash, Y. S.
AU - Kannan, Mathur S
AU - Sieck, Gary C.
PY - 1997/4
Y1 - 1997/4
N2 - With real-time confocal microscopy, the effect of three nitric oxide (NO) donors, S-nitroso-N-acetylpenicillamine, S-nitrosoglutathione, and diethylamine NO adduct, on the dynamic intracellular Ca2+ concentration ([Ca2+](i)) response of porcine tracheal smooth muscle (TSM) cells to acetylcholine (ACh) was examined. ACh initiated propagating [Ca2+](i) oscillations in TSM cells, which were inhibited by NO donors. 8- Bromoguanosine 3',5'-cyclic monophosphate slowed the frequency of [Ca2+](i) oscillations but did not completely inhibit oscillations, suggesting that the effects of NO donors are only partially mediated via guanosine 3',5'-cyclic monophosphate-dependent mechanisms. After preexposure to NO donors, ACh induced a small biphasic [Ca2+](i) response that was blocked by nifedipine, suggesting a lack of effect on Ca2+ influx through voltage-gated channels. In addition, NO donors did not inhibit Ca2+ influx induced by BAY K 8644. The [Ca2+](i) response to caffeine was inhibited by NO donors, indicating inhibition of sarcoplasmic reticulum (SR) Ca2+ release. When Ca2+ influx and SR Ca2+ reuptake were blocked, basal [Ca2+](i) increased, and this was inhibited by NO donors, suggesting enhanced Ca2+ efflux. These results indicate that NO donors inhibit [Ca2+](i) oscillations by blocking SR Ca2+ release and enhancing Ca2+ extrusion.
AB - With real-time confocal microscopy, the effect of three nitric oxide (NO) donors, S-nitroso-N-acetylpenicillamine, S-nitrosoglutathione, and diethylamine NO adduct, on the dynamic intracellular Ca2+ concentration ([Ca2+](i)) response of porcine tracheal smooth muscle (TSM) cells to acetylcholine (ACh) was examined. ACh initiated propagating [Ca2+](i) oscillations in TSM cells, which were inhibited by NO donors. 8- Bromoguanosine 3',5'-cyclic monophosphate slowed the frequency of [Ca2+](i) oscillations but did not completely inhibit oscillations, suggesting that the effects of NO donors are only partially mediated via guanosine 3',5'-cyclic monophosphate-dependent mechanisms. After preexposure to NO donors, ACh induced a small biphasic [Ca2+](i) response that was blocked by nifedipine, suggesting a lack of effect on Ca2+ influx through voltage-gated channels. In addition, NO donors did not inhibit Ca2+ influx induced by BAY K 8644. The [Ca2+](i) response to caffeine was inhibited by NO donors, indicating inhibition of sarcoplasmic reticulum (SR) Ca2+ release. When Ca2+ influx and SR Ca2+ reuptake were blocked, basal [Ca2+](i) increased, and this was inhibited by NO donors, suggesting enhanced Ca2+ efflux. These results indicate that NO donors inhibit [Ca2+](i) oscillations by blocking SR Ca2+ release and enhancing Ca2+ extrusion.
KW - acetylcholine
KW - airway
KW - confocal microscopy
KW - fluorescence
KW - guanylyl cyclase
KW - sarcoplasmic reticulum
KW - second messenger
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U2 - 10.1152/ajplung.1997.272.4.l588
DO - 10.1152/ajplung.1997.272.4.l588
M3 - Article
C2 - 9142929
AN - SCOPUS:0030961597
VL - 272
SP - L588-L596
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
SN - 0363-6143
IS - 4 16-4
ER -