Nine new Candida species near C. membranifaciens isolated from insects

Sung Oui Suh, Nhu H. Nguyen, Meredith Blackwell

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52 Scopus citations

Abstract

14 different yeasts were isolated from the gut of a variety of insects, including beetles, lacewings, fishflies, craneflies, and a cockroach. One of the yeasts was found both in the gut and on the body surface of a beetle larva. Based on ribosomal DNA sequence comparisons and phenotypic characters, the yeasts were identified as Candida membranifaciens, C. tenuis, Pichia nakazawae, and nine undescribed taxa in Saccharomycotina. All the undescribed taxa reproduced only asexually, and they fit within the limits of the polyphyletic genus Candida. The new species and their type strains are Candida blattariae NRRL Y-27703T, C. amphixiae NRRL Y-27704T, C. michaelii NRRL Y-27705T, C. cerambycidarum NRRL Y-27706T, C. gorgasii NRRL Y-27707T, C. endomychidarum NRRL Y-27708T, C. temnochilae NRRL Y-27763T, C. sinolaborantium NRRL Y-27765 T, and C. lessepsii NRRL Y-27766T spp. nov. Phylogenetic analysis of combined small and large subunit ribosomal DNA sequences placed C. amphixiae, C. michaelii, C. cerambycidarum, C. gorgasii, C. endomychidarum, and C. lessepsii in a statistically well supported clade with C. blattariae, C. membranifaciens, C. friedrichii, and C. buinensis as sisters to the clade. The other two new taxa, C. temnochilae and C. sinolaborantium, formed an independent clade basal to the major clade containing C. membranifaciens and closely related taxa. C. sinolaborantium occurred in both Panama and the USA, but there were genetic differences between the isolates from the two places.

Original languageEnglish (US)
Pages (from-to)1045-1056
Number of pages12
JournalMycological Research
Volume109
Issue number9
DOIs
StatePublished - Sep 2005

Bibliographical note

Funding Information:
We thank Cennet Erbil for her assistance during this study. Our colleague in a joint study, Joseph V. McHugh, helped to collect and identify the beetles. Once again we acknowledge the support of Donald Windsor, Oris Acevedo, and Maria Leone for graciously helping with logistics for collecting at the Smithsonian Tropical Research Institute, Barro Colorado Island, Panama. The staff of the M.D. Socolofsky Microscopy Center, LSU, graciously contributed microscopic expertise during this study. We acknowledge the curators and culture collections that preserve the germ plasm and data derived from and used in our studies: NRRL (Cletus Kurtzman) and CBS (Teun Boekhout and Vincent Robert). Use of the GenBank public database also is acknowledged. Our work was supported by the National Science Foundation, Biodiversity Surveys and Inventories Program (NSF DEB-0072741 and DEB-0417180), and REU supplements for support of undergraduate student Nhu H. Nguyen. The Louisiana State University Boyd Professor Fund also is acknowledged.

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