NGF/PI3K signaling-mediated epigenetic regulation of delta opioid receptor gene expression

Yulong L. Chen; Ping-Yee Law; Horace H Loh

doi:10.1016/j.bbrc.2008.01.164

NGF/PI3K signaling-mediated epigenetic regulation of delta opioid receptor gene expression

Yulong L. Chen, Ping-Yee Law, Horace H Loh

Pharmacology

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

The G protein-coupled delta opioid receptor gene (dor) has been associated with neuronal survival, differentiation, and neuroprotection. Our previous study identified PI3K/Akt/NF-κB signaling is a main downstream signaling pathway in nerve growth factor (NGF)-induced temporal expression of the dor gene in the PC12 cell model. It is still unknown how NGF/PI3K signaling regulates the expression of the dor gene in the nucleus. In the current study, we investigated how PI3K signaling affected epigenetic modifications of histone H3 Lys⁹ (H3K9) in the 5′-UTR region of the rat dor gene locus. NGF treatment resulted in the global reversal of H3K9 trimethylation in cells. Moreover, the locus-specific reversal of H3K9 trimethylation and acetylation of H3K9 were dependent upon NGF/PI3K signaling and temporally well correlated with NGF-induced gene expression. These results indicate the importance of epigenetic modifications of H3K9, particularly the reversal of trimethylated H3K9, in the regulation of NGF/PI3K-dependent genes during neuronal differentiation.

Original language	English (US)
Pages (from-to)	755-760
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	368
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2008.01.164
State	Published - Apr 11 2008

Bibliographical note

Funding Information:
This work was supported by National Institute of Health Grants DA-000546, DA-001583, DA-001806, DA07339, KO5-DA-70554 (HHL), K05-DA00513 (PYL), and by A. & F. Stark Fund of the Minnesota Medical Foundation.

Keywords

Akt
Chromatin remodeling
Delta opioid receptor
Histone modification
Lysine trimethylation
NF-κB
NGF
PI3K

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title = "NGF/PI3K signaling-mediated epigenetic regulation of delta opioid receptor gene expression",

abstract = "The G protein-coupled delta opioid receptor gene (dor) has been associated with neuronal survival, differentiation, and neuroprotection. Our previous study identified PI3K/Akt/NF-κB signaling is a main downstream signaling pathway in nerve growth factor (NGF)-induced temporal expression of the dor gene in the PC12 cell model. It is still unknown how NGF/PI3K signaling regulates the expression of the dor gene in the nucleus. In the current study, we investigated how PI3K signaling affected epigenetic modifications of histone H3 Lys9 (H3K9) in the 5′-UTR region of the rat dor gene locus. NGF treatment resulted in the global reversal of H3K9 trimethylation in cells. Moreover, the locus-specific reversal of H3K9 trimethylation and acetylation of H3K9 were dependent upon NGF/PI3K signaling and temporally well correlated with NGF-induced gene expression. These results indicate the importance of epigenetic modifications of H3K9, particularly the reversal of trimethylated H3K9, in the regulation of NGF/PI3K-dependent genes during neuronal differentiation.",

keywords = "Akt, Chromatin remodeling, Delta opioid receptor, Histone modification, Lysine trimethylation, NF-κB, NGF, PI3K",

author = "Chen, {Yulong L.} and Ping-Yee Law and Loh, {Horace H}",

note = "Funding Information: This work was supported by National Institute of Health Grants DA-000546, DA-001583, DA-001806, DA07339, KO5-DA-70554 (HHL), K05-DA00513 (PYL), and by A. & F. Stark Fund of the Minnesota Medical Foundation.",

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AU - Loh, Horace H

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AB - The G protein-coupled delta opioid receptor gene (dor) has been associated with neuronal survival, differentiation, and neuroprotection. Our previous study identified PI3K/Akt/NF-κB signaling is a main downstream signaling pathway in nerve growth factor (NGF)-induced temporal expression of the dor gene in the PC12 cell model. It is still unknown how NGF/PI3K signaling regulates the expression of the dor gene in the nucleus. In the current study, we investigated how PI3K signaling affected epigenetic modifications of histone H3 Lys9 (H3K9) in the 5′-UTR region of the rat dor gene locus. NGF treatment resulted in the global reversal of H3K9 trimethylation in cells. Moreover, the locus-specific reversal of H3K9 trimethylation and acetylation of H3K9 were dependent upon NGF/PI3K signaling and temporally well correlated with NGF-induced gene expression. These results indicate the importance of epigenetic modifications of H3K9, particularly the reversal of trimethylated H3K9, in the regulation of NGF/PI3K-dependent genes during neuronal differentiation.

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NGF/PI3K signaling-mediated epigenetic regulation of delta opioid receptor gene expression

Abstract

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Keywords

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