NF-κB activation in T cells requires discrete control of IκB kinase α/β (IKKα/β) phosphorylation and IKKγ ubiquitination by the ADAP adapter protein

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Abstract

NF-κB activation following engagement of the antigen-specific T cell receptor involves protein kinase C-θ-dependent assembly of the CARMA1-BCL10-MALT1 (CBM) signalosome, which coordinates downstream activation of IκB kinase (IKK). We previously identified a novel role for the adhesion- and degranulation-promoting adapter protein (ADAP) in regulating the assembly of the CBM complex via an interaction of ADAP with CARMA1. In this study, we identify a novel site in ADAP that is critical for association with the TAK1 kinase. ADAP is critical for recruitment of TAK1 and the CBM complex, but not IKK, to protein kinase C-θ. ADAP is not required for TAK1 activation. Although both the TAK1 and the CARMA1 binding sites in ADAP are essential for IκBα phosphorylation and degradation and NF-κB nuclear translocation, only the TAK1 binding site in ADAP is necessary for IKK phosphorylation. In contrast, only the CARMA1 binding site in ADAP is required for ubiquitination of IKKγ. Thus, distinct sites within ADAP control two key activation responses that are required for NF-κB activation in T cells.

Original languageEnglish (US)
Pages (from-to)11100-11105
Number of pages6
JournalJournal of Biological Chemistry
Volume285
Issue number15
DOIs
StatePublished - Apr 9 2010

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Phosphorylation
T-cells
Ubiquitination
Phosphotransferases
Chemical activation
T-Lymphocytes
Proteins
Binding Sites
Protein Kinase C
T-Cell Antigen Receptor
Adhesion
Association reactions
Antigens
Degradation

Cite this

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title = "NF-κB activation in T cells requires discrete control of IκB kinase α/β (IKKα/β) phosphorylation and IKKγ ubiquitination by the ADAP adapter protein",
abstract = "NF-κB activation following engagement of the antigen-specific T cell receptor involves protein kinase C-θ-dependent assembly of the CARMA1-BCL10-MALT1 (CBM) signalosome, which coordinates downstream activation of IκB kinase (IKK). We previously identified a novel role for the adhesion- and degranulation-promoting adapter protein (ADAP) in regulating the assembly of the CBM complex via an interaction of ADAP with CARMA1. In this study, we identify a novel site in ADAP that is critical for association with the TAK1 kinase. ADAP is critical for recruitment of TAK1 and the CBM complex, but not IKK, to protein kinase C-θ. ADAP is not required for TAK1 activation. Although both the TAK1 and the CARMA1 binding sites in ADAP are essential for IκBα phosphorylation and degradation and NF-κB nuclear translocation, only the TAK1 binding site in ADAP is necessary for IKK phosphorylation. In contrast, only the CARMA1 binding site in ADAP is required for ubiquitination of IKKγ. Thus, distinct sites within ADAP control two key activation responses that are required for NF-κB activation in T cells.",
author = "Rupa Srivastava and Burbach, {Brandon J} and Yoji Shimizu",
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T1 - NF-κB activation in T cells requires discrete control of IκB kinase α/β (IKKα/β) phosphorylation and IKKγ ubiquitination by the ADAP adapter protein

AU - Srivastava, Rupa

AU - Burbach, Brandon J

AU - Shimizu, Yoji

PY - 2010/4/9

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N2 - NF-κB activation following engagement of the antigen-specific T cell receptor involves protein kinase C-θ-dependent assembly of the CARMA1-BCL10-MALT1 (CBM) signalosome, which coordinates downstream activation of IκB kinase (IKK). We previously identified a novel role for the adhesion- and degranulation-promoting adapter protein (ADAP) in regulating the assembly of the CBM complex via an interaction of ADAP with CARMA1. In this study, we identify a novel site in ADAP that is critical for association with the TAK1 kinase. ADAP is critical for recruitment of TAK1 and the CBM complex, but not IKK, to protein kinase C-θ. ADAP is not required for TAK1 activation. Although both the TAK1 and the CARMA1 binding sites in ADAP are essential for IκBα phosphorylation and degradation and NF-κB nuclear translocation, only the TAK1 binding site in ADAP is necessary for IKK phosphorylation. In contrast, only the CARMA1 binding site in ADAP is required for ubiquitination of IKKγ. Thus, distinct sites within ADAP control two key activation responses that are required for NF-κB activation in T cells.

AB - NF-κB activation following engagement of the antigen-specific T cell receptor involves protein kinase C-θ-dependent assembly of the CARMA1-BCL10-MALT1 (CBM) signalosome, which coordinates downstream activation of IκB kinase (IKK). We previously identified a novel role for the adhesion- and degranulation-promoting adapter protein (ADAP) in regulating the assembly of the CBM complex via an interaction of ADAP with CARMA1. In this study, we identify a novel site in ADAP that is critical for association with the TAK1 kinase. ADAP is critical for recruitment of TAK1 and the CBM complex, but not IKK, to protein kinase C-θ. ADAP is not required for TAK1 activation. Although both the TAK1 and the CARMA1 binding sites in ADAP are essential for IκBα phosphorylation and degradation and NF-κB nuclear translocation, only the TAK1 binding site in ADAP is necessary for IKK phosphorylation. In contrast, only the CARMA1 binding site in ADAP is required for ubiquitination of IKKγ. Thus, distinct sites within ADAP control two key activation responses that are required for NF-κB activation in T cells.

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