New technologies accelerate the exploration of non-coding RNAs in horticultural plants

Degao Liu, Ritesh Mewalal, Rongbin Hu, Gerald A. Tuskan, Xiaohan Yang

Research output: Contribution to journalReview articlepeer-review

53 Scopus citations

Abstract

Non-coding RNAs (ncRNAs), that is, RNAs not translated into proteins, are crucial regulators of a variety of biological processes in plants. While protein-encoding genes have been relatively well-annotated in sequenced genomes, accounting for a small portion of the genome space in plants, the universe of plant ncRNAs is rapidly expanding. Recent advances in experimental and computational technologies have generated a great momentum for discovery and functional characterization of ncRNAs. Here we summarize the classification and known biological functions of plant ncRNAs, review the application of next-generation sequencing (NGS) technology and ribosome profiling technology to ncRNA discovery in horticultural plants and discuss the application of new technologies, especially the new genome-editing tool clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPRassociated protein 9 (Cas9) systems, to functional characterization of plant ncRNAs.

Original languageEnglish (US)
Article number31
JournalHorticulture Research
Volume4
DOIs
StatePublished - Jul 5 2017
Externally publishedYes

Bibliographical note

Funding Information:
We thank Dr Zong-Ming Cheng (University of Tennessee) and the reviewers for their critical review and instructive comments on our manuscript. This research is supported by the Department of Energy (DOE), Office of Science, Genomic Science Program under Award Number DE-SC0008834. Oak Ridge National Laboratory is managed by UT-Battelle, LLC for the US DOE under Contract Number DE-AC05- 00OR22725. This manuscript has been authored by UT-Battelle, LLC under Contract No. DE-AC05-00OR22725 with the U.S. Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan).

Publisher Copyright:
© The Author(s) 2017.

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