Genes on the long arm of the Drosophila melanogaster 4th chromosome are difficult to study because the chromosome lacks mitotic and meiotic recombination. Without recombination numerous standard methods of genetic analysis are impossible. Here, we report new resources for the 4th. For mitotic recombination, we generated a chromosome with an FRT very near the centromere in 101F and a derivative that carries FRT101F with a distal ubiquitously expressed GAL80 transgene. This pair of chromosomes enables both unmarked and MARCM clones. For meiotic recombination, we demonstrate that a Bloom syndrome helicase and recombination defective double mutant genotype can create recombinant 4th chromosomes via female meiosis. All strains will be available to the community via the Bloomington Drosophila Stock Center. Additional resources for studies of the 4th are in preparation and will also be made available. The goal of the 4th Chromosome Resource Project is to accelerate the genetic analysis of protein-coding genes on the 4th, including the 44 genes with no demonstrated function. Studies of these previously inaccessible but largely conserved genes will close longstanding gaps in our knowledge of metazoan development and physiology.
Bibliographical noteFunding Information:
The authors thank Jeff Sekelsky for flies and guidance on the Blm phenotype. The authors thank the Bloomington Stock Center and Shu Kondo for flies, Pierre Leopold and Carine Ganem-Elbaz for antibodies, and Kevin Cook for advice on Ubx phenotypes. The authors also thank Mike Stinchfield, Manisha Warrier, and Robert Wisotzkey for assistance with flies and bioinformatics. The Shimmi lab was supported by the Sigrid Juselius Foundation, the O’Connor lab by NIH GM118029, the Newfeld lab by NIH OD024794-S1 and the Newfeld/O’Connor labs by NIH OD024794 and OD028242.
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- adult brain
PubMed: MeSH publication types
- Journal Article
- Research Support, N.I.H., Extramural
- Research Support, Non-U.S. Gov't