New mammalian chloride channel identified by expression cloning

Markus Paulmichl, Yi Li, Kevin Wickman, Michael Ackerman, Ernest Peralta, David Clapham

Research output: Contribution to journalArticlepeer-review

298 Scopus citations

Abstract

ION channels selectively permeable to chloride ions regulate cell functions as diverse as excitability and control of cell volume1-5. Using expression cloning techniques, a complementary DNA from an epithelial cell line has been isolated, sequenced and its putative structure examined by site-directed mutagenesis. This cDNA, encoding a 235-amino-acid protein, gave rise to a chloride-selective outward current when expressed in oocytes. The expressed, outwardly rectifying chloride current was calcium-insensitive and was blocked by nucleotides applied to the cell surface. Mutation of a putative nucleotide-binding site resulted in loss of nucleotide block but incurred dependence on extracellular calcium concentration. The unusual sequence of this putative channel protein suggests a new class of ion channels not related to other previously cloned chloride channels6-11.

Original languageEnglish (US)
Pages (from-to)238-241
Number of pages4
JournalNature
Volume356
Issue number6366
DOIs
StatePublished - Jan 1 1992

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