New Aequorea Fluorescent Proteins for Cell-Free Bioengineering

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2 Scopus citations

Abstract

Recently, a new subset of fluorescent proteins has been identified from the Aequorea species of jellyfish. These fluorescent proteins were characterized in vivo; however, there has not been validation of these proteins within cell-free systems. Cell-free systems and technology development is a rapidly expanding field, encompassing foundational research, synthetic cells, bioengineering, biomanufacturing, and drug development. Cell-free systems rely heavily on fluorescent proteins as reporters. Here we characterize and validate this new set of Aequorea proteins for use in a variety of cell-free and synthetic cell expression platforms.

Original languageEnglish (US)
Pages (from-to)1371-1376
Number of pages6
JournalACS Synthetic Biology
Volume12
Issue number4
DOIs
StatePublished - Apr 21 2023

Bibliographical note

Funding Information:
We thank Dr. Nathan Shaner for the gift of plasmids containing the new fluorescent proteins tested in this work. This work was supported by NSF award 1807461 SeMiSynBio Very Large scale genetic circuit design and automation, NSF award 1840301 RoL:FELS:RAISE Building and Modeling Synthetic Bacterial Cells, NSF award 2123465 Synthetic P-bodies: Coupling gene expression and ribonucleoprotein granules in synthetic cell vesicles for sensing and response. Wakana Sato was supported by the Funai Overseas Scholarship of The Funai Foundation for Information Technology.

Publisher Copyright:
© 2023 American Chemical Society.

Keywords

  • Aequorea fluorescent proteins
  • cell-free translation
  • synthetic cells

PubMed: MeSH publication types

  • Journal Article
  • Research Support, U.S. Gov't, Non-P.H.S.

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