Neurotoxicity in rats chronically exposed to lead ingestion: Measurement of intracellular concentrations of free calcium and lead ions in resting or depolarized brain slices

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Abstract

The intracellular free calcium (Ca2+) or lead (Pb(i)2+) ions were measured by using 19F-NMR and 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid in brain slices obtained from control rats and rats chronically exposed to 1 mg/kg.day or 100 mg/kg.day dose of Pb acetate ingestion. The basal Ca(i)2+ values obtained in this study were comparable to the values reported previously for brain neurons by using the Fura-2 method (Singh, 1993a). The concentrations of total Pb in blood samples obtained from 2-week old rats exposed to 1 and 100 mg/kg.day doses of lead acetate were 31 and 521 μg/dl respectively. The concentrations of total Pb in brain samples obtained from 2-week old rats exposed to 1 and 100 mg/kg.day doses of lead acetate were 150 and 2700 ng/g respectively. The concentration of Pb in neonatal and adult samples did not differ significantly. Free Pb2+ was not detected in samples obtained from the rats exposed to a Pb dose of 1 mg/kg.day; however, a small concentration (ranging from 100 to 300 pM) of the ion was detected in samples obtained from the rats exposed to a Pb dose of 100 mg/kg.day. This suggests that either a small proportion of total Pb is ionized in brain or there is an efflux of Pb2+ from intracellular fluid into the incubation medium. Depolarization caused significant increase in Ca(i)2+ in cortical slices obtained both from control and Pb-exposed rats. However, depolarization increased Pb(i)2+ only in samples obtained from rats exposed to a Pb dose of 100 mg/kg.day. Chronic Pb exposure significantly reduced the effects of depolarization on Ca(i)2+ levels. Since there is very little extracellular Pb2+ remaining in the slices, the observed decrease in depolarization-induced Ca(i)2+ may not be because of the inhibition by Pb2+ in the influx of Ca2+ but because of long-term changes in cellular metabolism.

Original languageEnglish (US)
Pages (from-to)133-138
Number of pages6
JournalNeuroToxicology
Volume16
Issue number1
StatePublished - Jan 1 1995

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Rats
Brain
Eating
Ions
Calcium
Depolarization
Rat control
Intracellular Fluid
Ethane
Fura-2
Metabolism
Neurons
Acetates
Blood
Nuclear magnetic resonance
Acids
Fluids

Keywords

  • brain slices
  • depolarization
  • intracellular calcium
  • intracellular lead
  • NMR

Cite this

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title = "Neurotoxicity in rats chronically exposed to lead ingestion: Measurement of intracellular concentrations of free calcium and lead ions in resting or depolarized brain slices",
abstract = "The intracellular free calcium (Ca2+) or lead (Pb(i)2+) ions were measured by using 19F-NMR and 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid in brain slices obtained from control rats and rats chronically exposed to 1 mg/kg.day or 100 mg/kg.day dose of Pb acetate ingestion. The basal Ca(i)2+ values obtained in this study were comparable to the values reported previously for brain neurons by using the Fura-2 method (Singh, 1993a). The concentrations of total Pb in blood samples obtained from 2-week old rats exposed to 1 and 100 mg/kg.day doses of lead acetate were 31 and 521 μg/dl respectively. The concentrations of total Pb in brain samples obtained from 2-week old rats exposed to 1 and 100 mg/kg.day doses of lead acetate were 150 and 2700 ng/g respectively. The concentration of Pb in neonatal and adult samples did not differ significantly. Free Pb2+ was not detected in samples obtained from the rats exposed to a Pb dose of 1 mg/kg.day; however, a small concentration (ranging from 100 to 300 pM) of the ion was detected in samples obtained from the rats exposed to a Pb dose of 100 mg/kg.day. This suggests that either a small proportion of total Pb is ionized in brain or there is an efflux of Pb2+ from intracellular fluid into the incubation medium. Depolarization caused significant increase in Ca(i)2+ in cortical slices obtained both from control and Pb-exposed rats. However, depolarization increased Pb(i)2+ only in samples obtained from rats exposed to a Pb dose of 100 mg/kg.day. Chronic Pb exposure significantly reduced the effects of depolarization on Ca(i)2+ levels. Since there is very little extracellular Pb2+ remaining in the slices, the observed decrease in depolarization-induced Ca(i)2+ may not be because of the inhibition by Pb2+ in the influx of Ca2+ but because of long-term changes in cellular metabolism.",
keywords = "brain slices, depolarization, intracellular calcium, intracellular lead, NMR",
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year = "1995",
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pages = "133--138",
journal = "NeuroToxicology",
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T2 - Measurement of intracellular concentrations of free calcium and lead ions in resting or depolarized brain slices

AU - Singh, Ashok K

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N2 - The intracellular free calcium (Ca2+) or lead (Pb(i)2+) ions were measured by using 19F-NMR and 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid in brain slices obtained from control rats and rats chronically exposed to 1 mg/kg.day or 100 mg/kg.day dose of Pb acetate ingestion. The basal Ca(i)2+ values obtained in this study were comparable to the values reported previously for brain neurons by using the Fura-2 method (Singh, 1993a). The concentrations of total Pb in blood samples obtained from 2-week old rats exposed to 1 and 100 mg/kg.day doses of lead acetate were 31 and 521 μg/dl respectively. The concentrations of total Pb in brain samples obtained from 2-week old rats exposed to 1 and 100 mg/kg.day doses of lead acetate were 150 and 2700 ng/g respectively. The concentration of Pb in neonatal and adult samples did not differ significantly. Free Pb2+ was not detected in samples obtained from the rats exposed to a Pb dose of 1 mg/kg.day; however, a small concentration (ranging from 100 to 300 pM) of the ion was detected in samples obtained from the rats exposed to a Pb dose of 100 mg/kg.day. This suggests that either a small proportion of total Pb is ionized in brain or there is an efflux of Pb2+ from intracellular fluid into the incubation medium. Depolarization caused significant increase in Ca(i)2+ in cortical slices obtained both from control and Pb-exposed rats. However, depolarization increased Pb(i)2+ only in samples obtained from rats exposed to a Pb dose of 100 mg/kg.day. Chronic Pb exposure significantly reduced the effects of depolarization on Ca(i)2+ levels. Since there is very little extracellular Pb2+ remaining in the slices, the observed decrease in depolarization-induced Ca(i)2+ may not be because of the inhibition by Pb2+ in the influx of Ca2+ but because of long-term changes in cellular metabolism.

AB - The intracellular free calcium (Ca2+) or lead (Pb(i)2+) ions were measured by using 19F-NMR and 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid in brain slices obtained from control rats and rats chronically exposed to 1 mg/kg.day or 100 mg/kg.day dose of Pb acetate ingestion. The basal Ca(i)2+ values obtained in this study were comparable to the values reported previously for brain neurons by using the Fura-2 method (Singh, 1993a). The concentrations of total Pb in blood samples obtained from 2-week old rats exposed to 1 and 100 mg/kg.day doses of lead acetate were 31 and 521 μg/dl respectively. The concentrations of total Pb in brain samples obtained from 2-week old rats exposed to 1 and 100 mg/kg.day doses of lead acetate were 150 and 2700 ng/g respectively. The concentration of Pb in neonatal and adult samples did not differ significantly. Free Pb2+ was not detected in samples obtained from the rats exposed to a Pb dose of 1 mg/kg.day; however, a small concentration (ranging from 100 to 300 pM) of the ion was detected in samples obtained from the rats exposed to a Pb dose of 100 mg/kg.day. This suggests that either a small proportion of total Pb is ionized in brain or there is an efflux of Pb2+ from intracellular fluid into the incubation medium. Depolarization caused significant increase in Ca(i)2+ in cortical slices obtained both from control and Pb-exposed rats. However, depolarization increased Pb(i)2+ only in samples obtained from rats exposed to a Pb dose of 100 mg/kg.day. Chronic Pb exposure significantly reduced the effects of depolarization on Ca(i)2+ levels. Since there is very little extracellular Pb2+ remaining in the slices, the observed decrease in depolarization-induced Ca(i)2+ may not be because of the inhibition by Pb2+ in the influx of Ca2+ but because of long-term changes in cellular metabolism.

KW - brain slices

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KW - intracellular calcium

KW - intracellular lead

KW - NMR

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