Neurons in the sacral parasympathetic nucleus that project to the hypothalamus do not also project through the pelvic nerve-a double labeling study combining Fluoro-gold and cholera toxin B in the rat

Rami Burstein, Jianlin Wang, Robert P. Elde, Glenn J Giesler

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22 Scopus citations

Abstract

We recently reported that neurons in the sacral parasympathetic nucleus (SPN) project directly to the hypothalamus. In the present study, we examined the possibility that individual neurons in SPN send both an axon into the hypothalamus. We used a new double-labeling technique in which two sensitive retrograde tracers (Fluoro-gold and cholera toxin subunit B immunocytochemically stained with rhodamine-labeled antibodies) were combined. The effectiveness of this combination for singly and doubly labeling neurons was established in experiments in which both tracers were injected into overlapping areas of the tongue or ventrobasal thalamus. These injections doubly labeled large numbers of neurons in the hypoglossal or dorsal column nuclei, respectively. In studies of the projections of neurons in the SPN, injection of one tracer into the hypothalamus and the other into the pelvic nerve and/or pelvic ganglion singly labeled many neurons (more than 3300 in the 7 examined cases). However, no SPN neurons were doubly labeled. These findings indicate that the SPN in the rat consists of at least two distinct groups of cells, parasympathetic preganglionic neurons and neurons that project to the hypothalamus.

Original languageEnglish (US)
Pages (from-to)159-165
Number of pages7
JournalBrain Research
Volume506
Issue number1
DOIs
StatePublished - Jan 1 1990

Keywords

  • Cholera toxin B
  • Double labeling
  • Fluoro-gold
  • Hypothalamus
  • Pelvic nerve
  • Pelvic viscera
  • Sacral parasympathetic nucleus, Spinohypothalamic tract

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