Neuromodulation of enteropathogen internalization in Peyer's patches from porcine jejunum

Benedict T. Green, Mark Lyte, Anjali Kulkarni-Narla, David R. Brown

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Jejunal Peyer's patches (JPP) are innervated sites of immune induction and enteropathogen infection. We investigated the role of enteric nerves in modulating pathogen entry into porcine JPP. Presumptive norepinephrine (NE)-containing nerve fibers were localized in JPP domes and follicle-associated villi by secondary immunofluorescence histochemistry. NE or the neuronal conduction blocker saxitoxin increased intracellular internalization of pathogenic Salmonella choleraesuis and Escherichia coli O157:H7, but not nonpathogenic E. coli, into isolated JPP mucosa. NE action was prevented by the α-adrenergic antagonist phentolamine. Withdrawal of enteric neural activity or NE administration appears to modulate JPP interactions with pathogenic bacteria.

Original languageEnglish (US)
Pages (from-to)74-82
Number of pages9
JournalJournal of Neuroimmunology
Volume141
Issue number1-2
DOIs
StatePublished - Aug 2003

Keywords

  • Enteric nervous system
  • Enterohemorrhagic Escherichia coli
  • Mucosal immunity
  • Salmonella enterica serovar choleraesuis
  • Sympathetic nerves

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