Neuromedin U octapeptide alters ion transport in porcine jejunum

David R. Brown; Francis L. Quito

doi:10.1016/0014-2999(88)90415-3

Neuromedin U octapeptide alters ion transport in porcine jejunum

David R. Brown
, Francis L. Quito

Chronic Disease Biology

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

The gut neuropeptide, neuromedin U octapeptide (NMU-8) produced transient elevations in short-circuit current (EC₅₀ = 0.7 nmol/1) after its contraluminal administration to sheets of porcine distal jejunal mucosa in vitro. Mucosal responses to NMU-8 were unaffected by 1 μmol/1 atropine or hexamethonium, but were abolished by tetrodotoxin (0.1 μmol/l), dependent upon extracellular Cl, and underwent tachyphylaxis upon repeated peptide application. NMU-8 did not affect contractility of isolated jejunal longitudinal muscle. Thus, NMU-8 appears to selectively modify intestinal ion transport through interactions with non-cholinergic enteric neurons.

Original language	English (US)
Pages (from-to)	159-162
Number of pages	4
Journal	European Journal of Pharmacology
Volume	155
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-2999(88)90415-3
State	Published - Oct 11 1988

Bibliographical note

Funding Information:
The authors thank Ward A. Ortman for excellent technical assistance in performing smooth muscle bioassays. This work was funded in part by Minnesota Agricultural Experiment Station Grant MN-061-37 and National Institutes of Health Grant DK-37497.

Keywords

(Longitudinal muscle, Contractility, Pig)
Enteric nervous system
Intestinal ion transport
Jejunum (distal)
Neuromedin U

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10.1016/0014-2999(88)90415-3

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@article{634df04045ff4e54993617bc3203218d,

title = "Neuromedin U octapeptide alters ion transport in porcine jejunum",

abstract = "The gut neuropeptide, neuromedin U octapeptide (NMU-8) produced transient elevations in short-circuit current (EC50 = 0.7 nmol/1) after its contraluminal administration to sheets of porcine distal jejunal mucosa in vitro. Mucosal responses to NMU-8 were unaffected by 1 μmol/1 atropine or hexamethonium, but were abolished by tetrodotoxin (0.1 μmol/l), dependent upon extracellular Cl, and underwent tachyphylaxis upon repeated peptide application. NMU-8 did not affect contractility of isolated jejunal longitudinal muscle. Thus, NMU-8 appears to selectively modify intestinal ion transport through interactions with non-cholinergic enteric neurons.",

keywords = "(Longitudinal muscle, Contractility, Pig), Enteric nervous system, Intestinal ion transport, Jejunum (distal), Neuromedin U",

author = "Brown, \{David R.\} and Quito, \{Francis L.\}",

note = "Funding Information: The authors thank Ward A. Ortman for excellent technical assistance in performing smooth muscle bioassays. This work was funded in part by Minnesota Agricultural Experiment Station Grant MN-061-37 and National Institutes of Health Grant DK-37497.",

year = "1988",

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doi = "10.1016/0014-2999(88)90415-3",

language = "English (US)",

volume = "155",

pages = "159--162",

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T1 - Neuromedin U octapeptide alters ion transport in porcine jejunum

AU - Brown, David R.

AU - Quito, Francis L.

N1 - Funding Information: The authors thank Ward A. Ortman for excellent technical assistance in performing smooth muscle bioassays. This work was funded in part by Minnesota Agricultural Experiment Station Grant MN-061-37 and National Institutes of Health Grant DK-37497.

PY - 1988/10/11

Y1 - 1988/10/11

N2 - The gut neuropeptide, neuromedin U octapeptide (NMU-8) produced transient elevations in short-circuit current (EC50 = 0.7 nmol/1) after its contraluminal administration to sheets of porcine distal jejunal mucosa in vitro. Mucosal responses to NMU-8 were unaffected by 1 μmol/1 atropine or hexamethonium, but were abolished by tetrodotoxin (0.1 μmol/l), dependent upon extracellular Cl, and underwent tachyphylaxis upon repeated peptide application. NMU-8 did not affect contractility of isolated jejunal longitudinal muscle. Thus, NMU-8 appears to selectively modify intestinal ion transport through interactions with non-cholinergic enteric neurons.

AB - The gut neuropeptide, neuromedin U octapeptide (NMU-8) produced transient elevations in short-circuit current (EC50 = 0.7 nmol/1) after its contraluminal administration to sheets of porcine distal jejunal mucosa in vitro. Mucosal responses to NMU-8 were unaffected by 1 μmol/1 atropine or hexamethonium, but were abolished by tetrodotoxin (0.1 μmol/l), dependent upon extracellular Cl, and underwent tachyphylaxis upon repeated peptide application. NMU-8 did not affect contractility of isolated jejunal longitudinal muscle. Thus, NMU-8 appears to selectively modify intestinal ion transport through interactions with non-cholinergic enteric neurons.

KW - (Longitudinal muscle, Contractility, Pig)

KW - Enteric nervous system

KW - Intestinal ion transport

KW - Jejunum (distal)

KW - Neuromedin U

UR - https://www.scopus.com/pages/publications/0023801587

UR - https://www.scopus.com/pages/publications/0023801587#tab=citedBy

U2 - 10.1016/0014-2999(88)90415-3

DO - 10.1016/0014-2999(88)90415-3

M3 - Article

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AN - SCOPUS:0023801587

SN - 0014-2999

VL - 155

SP - 159

EP - 162

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 1-2

ER -

Neuromedin U octapeptide alters ion transport in porcine jejunum

Abstract

Bibliographical note

Keywords

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