Neurochemical correlates of synapse density in a Huntington's disease mouse model

Nicole L Zarate, Katherine Gundry, Dahyun Yu, Jordan Casby, Lynn E. Eberly, Gülin Öz, Rocio Gomez-Pastor

Research output: Contribution to journalArticlepeer-review

Abstract

Striatal medium spiny neurons are highly susceptible in Huntington's disease (HD), resulting in progressive synaptic perturbations that lead to neuronal dysfunction and death. Non-invasive imaging techniques, such as proton magnetic resonance spectroscopy ( 1 H-MRS), are used in HD mouse models and patients with HD to monitor neurochemical changes associated with neuronal health. However, the association between brain neurochemical alterations and synaptic dysregulation remains unknown, limiting our ability to monitor potential treatments that may affect synapse function. We conducted in vivo longitudinal 1 H-MRS in the striatum followed by ex vivo analyses of excitatory synapse density of two synaptic circuits disrupted in HD, thalamo-striatal (T-S), and cortico-striatal (C-S) pathways, to assess the relationship between neurochemical alterations and changes in synapse density. We used the zQ175 (Tg/0) HD mouse model as well as zQ175 mice lacking one allele of CK2α'(zQ175 (Tg/0) :CK2α' (+/-) ), a kinase previously shown to regulate synapse function in HD. Longitudinal analyses of excitatory synapse density showed early and sustained reduction in T-S synapses in zQ175 mice, preceding C-S synapse depletion, which was rescued in zQ175:CK2α' (+/-) . Changes in T-S and C-S synapses were accompanied by progressive alterations in numerous neurochemicals between WT and HD mice. Linear regression analyses showed C-S synapse number positively correlated with 1 H-MRS-measured levels of GABA, while T-S synapse number positively correlated with levels of phosphoethanolamine and negatively correlated with total creatine levels. These associations suggest that these neurochemical concentrations measured by 1 H-MRS may facilitate monitoring circuit-specific synaptic dysfunction in the zQ175 mouse model and in other HD pre-clinical studies.

Original languageEnglish (US)
Pages (from-to)226-241
Number of pages16
JournalJournal of Neurochemistry
Volume164
Issue number2
DOIs
StatePublished - Jan 2023

Bibliographical note

Publisher Copyright:
© 2022 The Authors. Journal of Neurochemistry published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry.

Keywords

  • CK2 alpha prime
  • H-MRS
  • Huntington's disease
  • neurochemicals
  • synapse density
  • zQ175

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