Neurochemical and BOLD responses during neuronal activation measured in the human visual cortex at 7 Tesla

Petr Bednarik, Ivan Tkac, Federico Giove, Mauro Dinuzzo, Dinesh K Deelchand, Uzay E. Emir, Lynn E Eberly, Silvia Mangia

Research output: Contribution to journalArticle

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Abstract

Several laboratories have consistently reported small concentration changes in lactate, glutamate, aspartate, and glucose in the human cortex during prolonged stimuli. However, whether such changes correlate with blood oxygenation level-dependent functional magnetic resonance imaging (BOLD-fMRI) signals have not been determined. The present study aimed at characterizing the relationship between metabolite concentrations and BOLD-fMRI signals during a block-designed paradigm of visual stimulation. Functional magnetic resonance spectroscopy (fMRS) and fMRI data were acquired from 12 volunteers. A short echo-time semi-LASER localization sequence optimized for 7 Tesla was used to achieve full signal-intensity MRS data. The group analysis confirmed that during stimulation lactate and glutamate increased by 0.26±0.06 μmol/g (∼30%) and 0.28±0.03 μmol/g (∼3%), respectively, while aspartate and glucose decreased by 0.20±0.04 μmol/g (∼5%) and 0.19±0.03 μmol/g (∼16%), respectively. The single-subject analysis revealed that BOLD-fMRI signals were positively correlated with glutamate and lactate concentration changes. The results show a linear relationship between metabolic and BOLD responses in the presence of strong excitatory sensory inputs, and support the notion that increased functional energy demands are sustained by oxidative metabolism. In addition, BOLD signals were inversely correlated with baseline γ-aminobutyric acid concentration. Finally, we discussed the critical importance of taking into account linewidth effects on metabolite quantification in fMRS paradigms.

Original languageEnglish (US)
Pages (from-to)601-610
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume35
DOIs
StatePublished - Mar 31 2015

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Visual Cortex
Magnetic Resonance Imaging
Glutamic Acid
Lactic Acid
Aspartic Acid
Magnetic Resonance Spectroscopy
Aminobutyrates
Glucose
Photic Stimulation
Volunteers

Keywords

  • Functional Spectroscopy
  • GABA quantification
  • Neurochemistry
  • Visual Stimulation

Cite this

Neurochemical and BOLD responses during neuronal activation measured in the human visual cortex at 7 Tesla. / Bednarik, Petr; Tkac, Ivan; Giove, Federico; Dinuzzo, Mauro; Deelchand, Dinesh K; Emir, Uzay E.; Eberly, Lynn E; Mangia, Silvia.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 35, 31.03.2015, p. 601-610.

Research output: Contribution to journalArticle

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