Abstract
Neuroblastoma X glioma NG108-15 hybrid cells cultured in a chemically defined medium within 3 cell passages, exhibited viability, growth rate and morphology similar to those of cells grown in medium supplemented with 5% fetal calf serum. Hybrid cells cultured in the chemically defined medium within these periods of time also did not exhibit a difference in basal adenylate cyclase activity nor in the enzymatic activities stimulated by adenosine, forskolin, NaF, GppNHp or Mn2+. Furthermore, opiate receptor density in chemically defined medium cultured cells remained identical to that in cells cultured in 5% fetal calf serum. The acute and chronic effects of opiates on adenylate cyclase were similar for cells grown under either set of conditions.
Original language | English (US) |
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Pages (from-to) | 370-373 |
Number of pages | 4 |
Journal | Brain Research |
Volume | 360 |
Issue number | 1-2 |
DOIs | |
State | Published - Dec 23 1985 |
Bibliographical note
Funding Information:The present research is supported in part by NIDA Grant DA 000564 and Army Contract DAMD17-84-C-4019. H.H.L. is a recipient of National Institute of Drug Abuse Career Award K02-DA-70554.
Keywords
- adenylate cyclase activity
- neuroblastoma X glioma NG108-15 hybrid cell
- opiate regulation
- serum-free chemically defined medium