Negative interactions between amino acid and methylamine/ammonia transport systems of Saccharomyces cerevisiae

R. J. Roon, J. S. Levy, F. Larimore

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The transport of methylamine (methylammonium ion) and ammonia (ammonium ion) is accomplished in S. cerevisiae by means of a specific active transport system. L Amino acids are noncompetitive inhibitors of methylamine transport. This inhibition is relieved or eliminated in mutant strains that have a reduced ability to transport amino acids. The inhibition of methylamine transport occurs immediately upon the addition of amino acids to the assay system and persists until the external amino acid pool is depleted. The degree of inhibition observed is a direct function of the rate of amino acid transport. Both methylamine and ammonia are capable of inhibiting amino acid transport. The inhibition of amino acid transport is eliminated in mutant strains that cannot transport methylamine and ammonia.

Original languageEnglish (US)
Pages (from-to)3599-3604
Number of pages6
JournalJournal of Biological Chemistry
Volume252
Issue number11
StatePublished - 1977

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