Nectin 4 overexpression in ovarian cancer tissues and serum: Potential role as a serum biomarker

Melissa S. DeRycke, Stefan E. Pambuccian, C. Blake Gilks, Steve E. Kalloger, Abderrezak Ghidouche, Marc Lopez, Robin L. Bliss, Melissa A Geller, Peter A Argenta, Katherine M. Harrington, Amy P Skubitz

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Early detection of ovarian cancer is difficult owing to the lack of specific and sensitive tests available. Previously, we found expression of nectin 4 to be increased in ovarian cancer compared with normal ovaries. Reverse transcriptase-polymerase chain reaction (RT-PCR) and quantitative RT-PCR validated the overexpression of nectin 4 messenger RNA in ovarian cancer compared with normal ovarian cell lines and tissues. Protein levels of nectin 4 were elevated in ovarian cancer cell lines and tissue compared with normal ovarian cell lines as demonstrated by Western immunoblotting, flow cytometry, and immunohistochemical staining of tissue microarray slides. Cleaved nectin 4 was detectable in a number of patient serum samples by enzymelinked immunosorbent assay. In patients with benign gynecologic diseases with high serum CA125 levels, nectin 4 was not detected in the majority of cases, suggesting that nectin 4 may serve as a potential biomarker that helps discriminate benign gynecologic diseases from ovarian cancer in a panel with CA125.

Original languageEnglish (US)
Pages (from-to)835-845
Number of pages11
JournalAmerican Journal of Clinical Pathology
Volume134
Issue number5
DOIs
StatePublished - Nov 1 2010

Fingerprint

Ovarian Neoplasms
Biomarkers
Serum
Female Genital Diseases
Reverse Transcriptase Polymerase Chain Reaction
Cell Line
Immunosorbents
Early Detection of Cancer
nectins
Ovary
Flow Cytometry
Western Blotting
Staining and Labeling
Messenger RNA
Proteins

Keywords

  • Detection
  • Diagnosis
  • Nectin 4
  • Ovarian cancer
  • Serum biomarker
  • Tissue microarray

Cite this

Nectin 4 overexpression in ovarian cancer tissues and serum : Potential role as a serum biomarker. / DeRycke, Melissa S.; Pambuccian, Stefan E.; Gilks, C. Blake; Kalloger, Steve E.; Ghidouche, Abderrezak; Lopez, Marc; Bliss, Robin L.; Geller, Melissa A; Argenta, Peter A; Harrington, Katherine M.; Skubitz, Amy P.

In: American Journal of Clinical Pathology, Vol. 134, No. 5, 01.11.2010, p. 835-845.

Research output: Contribution to journalArticle

DeRycke, MS, Pambuccian, SE, Gilks, CB, Kalloger, SE, Ghidouche, A, Lopez, M, Bliss, RL, Geller, MA, Argenta, PA, Harrington, KM & Skubitz, AP 2010, 'Nectin 4 overexpression in ovarian cancer tissues and serum: Potential role as a serum biomarker', American Journal of Clinical Pathology, vol. 134, no. 5, pp. 835-845. https://doi.org/10.1309/AJCPGXK0FR4MHIHB
DeRycke, Melissa S. ; Pambuccian, Stefan E. ; Gilks, C. Blake ; Kalloger, Steve E. ; Ghidouche, Abderrezak ; Lopez, Marc ; Bliss, Robin L. ; Geller, Melissa A ; Argenta, Peter A ; Harrington, Katherine M. ; Skubitz, Amy P. / Nectin 4 overexpression in ovarian cancer tissues and serum : Potential role as a serum biomarker. In: American Journal of Clinical Pathology. 2010 ; Vol. 134, No. 5. pp. 835-845.
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